Quantitative assessments of glycolysis from single cells

Autor:	Lisa Ta, Alex A. Dooraghi, Arion F. Chatziioannou, Michael E. Phelps, James R. Heath, Jungwoo Kim, Wilson X. Mai, Dazy Johnson, David Nathanson, Young Shik Shin
Rok vydání:	2015
Předmět:	medicine.diagnostic_test Cell Glucose analog Biology Molecular diagnostics Article In vitro Receptor tyrosine kinase medicine.anatomical_structure Biochemistry Positron emission tomography Cancer cell medicine Cancer research biology.protein Glycolysis
Zdroj:	TECHNOLOGY. :172-178
ISSN:	2345-7740 2339-5478
DOI:	10.1142/s2339547815200058
Popis:	The most common positron emission tomography (PET) radio-labeled probe for molecular diagnostics in patient care and research is the glucose analog, 2-deoxy-2-[F-18]fluoro-D-glucose (18F-FDG). We report on an integrated microfluidics-chip/beta particle imaging system for in vitro 18F-FDG radioassays of glycolysis with single cell resolution. We investigated the kinetic responses of single glioblastoma cancer cells to targeted inhibitors of receptor tyrosine kinase signaling. Further, we find a weak positive correlation between cell size and rate of glycolysis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5104e6cc413cef7ca27f4ac083254559 https://doi.org/10.1142/s2339547815200058 Zobrazit plný text záznamu