Use of the wound-inducible NtQPT2 promoter from Nicotiana tabacum for production of a plant-made vaccine
| Autor: | John D. Hamill, Diane Webster, Giorgio De Guzman, Amanda Maree Walmsley |
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| Rok vydání: | 2012 |
| Předmět: |
Transgene
Nicotiana tabacum Bacterial Toxins Bioengineering Genetically modified crops medicine.disease_cause Plant Roots Applied Microbiology and Biotechnology Enterotoxins Enterotoxigenic Escherichia coli Tobacco medicine Pentosyltransferases Promoter Regions Genetic Vaccines biology Toxin Escherichia coli Proteins General Medicine Plants Genetically Modified biology.organism_classification Molecular biology Quinolinate Genetically modified organism Biochemistry Cauliflower mosaic virus Biotechnology |
| Zdroj: | Biotechnology Letters. 34:1143-1150 |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/s10529-012-0879-6 |
| Popis: | The wound-inducible quinolinate phosphoribosyl transferase promoter from Nicotiana tabacum (NtQPT2) was assessed for its capacity to produce B-subunit of the heat-labile toxin (LTB) from enterotoxigenic Escherichia coli in transgenic plant tissues. Comparisons were made with the widely used and constitutive Cauliflower Mosaic Virus 35S (CaMV35S) promoter. The NtQPT2 promoter produced somewhat lower average concentrations of LTB protein per unit weight of hairy root tissue but allowed better growth thereby producing similar or higher overall average yields of LTB per culture batch. Transgenic tobacco plants containing the NtQPT2-LTB construct contained LTB protein in roots but not leaves. Moreover, wounding NtQPT2-LTB transgenic plants, by removal of apices, resulted in an approximate 500% increase in LTB levels in roots when analysed several days later. CaMV35S-LTB transgenic plants contained LTB protein in leaves and roots but wounding made no difference to their LTB content. |
| Databáze: | OpenAIRE |
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