Application of a marine luminescent Vibrio sp. B4L for biosynthesis of silver nanoparticles with unique characteristics, biochemical properties, antibacterial and antibiofilm activities
| Autor: | Noushin Zamanpour, Ahmad Kompany, Mirza Mohammad Reza Sharifmoghadam, Bahar Shahnavaz, Mansour Mashreghi, Ali Mohammad Esmaeily |
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| Rok vydání: | 2020 |
| Předmět: |
Staphylococcus aureus
Lysis Luminescence Silver Metal Nanoparticles Microbial Sensitivity Tests 01 natural sciences Biochemistry Silver nanoparticle Structure-Activity Relationship Marine bacteriophage Dynamic light scattering Drug Discovery Zeta potential Escherichia coli Molecular Biology Vibrio biology Dose-Response Relationship Drug Molecular Structure 010405 organic chemistry Chemistry Organic Chemistry Biofilm biology.organism_classification 0104 chemical sciences Anti-Bacterial Agents 010404 medicinal & biomolecular chemistry Biofilms Antibacterial activity Nuclear chemistry |
| Zdroj: | Bioorganic chemistry. 114 |
| ISSN: | 1090-2120 |
| Popis: | Biosynthesis of silver nanoparticles (AgNPs) by marine bacteria especially luminescent Vibrio species is least investigated. In this study, AgNPs were first synthesized by the culture supernatant of a luminescent bacterium (Vibrio sp. B4L) and then, the prepared samples were characterized employing several techniques. The antibacterial activity of the AgNPs was investigated against Escherichia coli and Staphylococcus aureus using disk diffusion agar and broth microdilution methods. The growth curve, Reactive Oxygen Species (ROS) formation, and Lactate Dehydrogenase (LDH) activity of the samples were measured along with Field Emission Scanning Electron Microscopy (FESEM) observation and inhibition of biofilm formation. Dynamic light scattering (DLS) analysis showed that the average particle size of the synthesized AgNPs was in the range of about 32.67–107.18 nm and the polydispersity index (PDI) of 0.1120 indicated the formation of monodispersed particles. The average zeta potential of AgNPs obtained −36.15 mV, showing the high stability of biosynthetic nanoparticles. Antibacterial studies indicated that not only the AgNPs had antibacterial activity but also increased the antibacterial properties of tetracycline when used in combination. ROS production was enhanced in a dose-dependent manner. A high difference in LDH activities was found between AgNPs treated cells and the control group. FESEM images revealed membrane disruption and lysis in AgNPs treated cells. The formation of E. coli biofilm was 100% inhibited at 62.5 µg/ml showing that our bacteriogenic AgNPs can be a potential alternative remedies for controlling antibiotic-resistant pathogens. |
| Databáze: | OpenAIRE |
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