Mixed‐solvent molecular dynamics simulation‐based discovery of a putative allosteric site on regulator of G protein signaling 4
| Autor: | Wallace K.B. Chan, Heather A. Carlson, Debarati DasGupta, John R. Traynor |
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| Rok vydání: | 2021 |
| Předmět: |
biology
Chemistry G protein Allosteric regulation Druggability General Chemistry Molecular Dynamics Simulation Phosphatidylinositols Small molecule Article G Protein-Coupled Receptor Signaling Cell biology RGS4 Computational Mathematics Drug Delivery Systems Regulator of G protein signaling Calmodulin Models Chemical Drug Design biology.protein Allosteric Site RGS Proteins Cysteine |
| Zdroj: | J Comput Chem |
| ISSN: | 1096-987X 0192-8651 |
| Popis: | Regulator of G protein signaling 4 (RGS4) is an intracellular protein that binds to the Gα subunit ofheterotrimeric G proteins and aids in terminating G protein coupled receptor signaling. RGS4 has been implicated in pain, schizophrenia, and the control of cardiac contractility. Inhibitors of RGS4 have been developed but bind covalently to cysteine residues on the protein. Therefore, we sought to identify alternative druggable sites on RGS4 using mixed-solvent molecular dynamics simulations, which employ low concentrations of organic probes to identify druggable hotspots on the protein. Pseudo-ligands were placed in consensus hotspots, and perturbation with normal mode analysis led to the identification and characterization of a putative allosteric site, which would be invaluable for structure-based drug design of non-covalent, small molecule inhibitors. Future studies on the mechanism of this allostery will aid in the development of novel therapeutics targeting RGS4. |
| Databáze: | OpenAIRE |
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