Long-term stability of clinically relevant chemistry analytes in pleural and peritoneal fluid
| Autor: | Adriana Unić, Lara Milevoj Kopcinovic, Jelena Culej, Marijana Miler, Marija Brčić, Alen Vrtarić, Nora Nikolac Gabaj, Marija Bozovic |
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| Rok vydání: | 2020 |
| Předmět: |
Male
stability pleural effusion peritoneal effusion preanalytical phase Time Factors Pleural effusion Clinical Biochemistry Peritoneal Effusion Andrology chemistry.chemical_compound Lactate dehydrogenase medicine Ascitic Fluid Humans Amylase Aged Aged 80 and over Blood Specimen Collection Creatinine biology Peritoneal fluid Biochemistry (medical) Albumin Middle Aged medicine.disease Original Papers Pleural Effusion chemistry Chemistry Clinical biology.protein Urea Female Blood Chemical Analysis |
| Zdroj: | Biochemia Medica Volume 30 Issue 2 |
| ISSN: | 1846-7482 1330-0962 |
| DOI: | 10.11613/bm.2020.020701 |
| Popis: | Introduction: Our aim was to investigate the stability of clinically relevant analytes in pleural and peritoneal fluids stored in variable time periods and variable storage temperatures prior to analysis. Materials and methods: Baseline total proteins (TP), albumin (ALB), lactate dehydrogenase (LD), cholesterol (CHOL), triglycerides (TRIG), creatinine (CREA), urea, glucose and amylase (AMY) were measured using standard methods in residual samples from 29 pleural and 12 peritoneal fluids referred to our laboratory. Aliquots were stored for 6 hours at room temperature (RT); 3, 7, 14 and 30 days at - 20°C. At the end of each storage period, all analytes were re-measured. Deviations were calculated and compared to stability limits (SL). Results: Pleural fluid TP and CHOL did not differ in the observed storage periods (P = 0.265 and P = 0.170, respectively). Statistically significant differences were found for ALB, LD, TRIG, CREA, urea, glucose and AMY. Peritoneal fluid TP, ALB, TRIG, urea and AMY were not statistically different after storage, contrary to LD, CHOL, CREA and glucose. Deviations for TP, ALB, CHOL, TRIG, CREA, urea and AMY in all storage periods tested for both serous fluids were within the SL. Deviations exceeding SL were observed for LD and glucose when stored for 3 and 7 days at - 20°C, respectively. Conclusions: TP, ALB, CHOL, TRIG, CREA, urea and AMY are stable in serous samples stored up to 6 hours at RT and/or 30 days at - 20°C. Glucose is stable up to 6 hours at RT and 3 days at - 20°C. The stability of LD in is limited to 6 hours at RT. |
| Databáze: | OpenAIRE |
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