Comparative proteomic analysis of human mesenchymal and embryonic stem cells: Towards the definition of a mesenchymal stem cell proteomic signature

Autor: Christian Jorgensen, Romain Barbet, Stéphane Roche, Bruno Delorme, Louis Casteilla, Danièle Noël, Pierre Charbord, Helen A. Papadaki, Jacques Hatzfeld, Karim Boumediene, Béatrice Cousin, Carole Crozet, Ollivier Milhavet, Robert A.J. Oostendorp, Sylvain Lehmann, David Caton
Přispěvatelé: Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Plateforme de Protéomique Clinique, CHU Saint-Eloi
Rok vydání: 2009
Předmět:
Proteomics
Umbilical Veins
Gene Expression
Clinical uses of mesenchymal stem cells
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
Biology
Stem cell marker
Biochemistry
03 medical and health sciences
0302 clinical medicine
Antigens
CD
Bone Marrow
Cancer stem cell
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
Humans
Electrophoresis
Gel
Two-Dimensional
Umbilical vein
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
Databases
Protein
Molecular Biology
Cells
Cultured
Embryonic Stem Cells
Mesenchymal stem cell
mass spectrometry
030304 developmental biology
Stem cell transplantation for articular cartilage repair
Analysis of Variance
0303 health sciences
Synovial Membrane
Proteins
Mesenchymal Stem Cells
Amniotic stem cells
3. Good health
Cell biology
Embryonic stem cell
Phenotype
2D-PAGE
Adipose Tissue
030220 oncology & carcinogenesis
Immunology
Stem cell
Adult stem cell
Zdroj: Proteomics
Proteomics, Wiley-VCH Verlag, 2009, 9 (2), pp.223-232. ⟨10.1002/pmic.200800035⟩
ISSN: 1615-9861
1615-9853
DOI: 10.1002/pmic.200800035
Popis: International audience; Mesenchymal stem cells (MSC) are adult multipotential progenitors which have a high potential in regenerative medicine. They can be isolated from different tissues throughout the body and their homogeneity in terms of phenotype and differentiation capacities is a real concern. To address this issue, we conducted a 2-DE gel analysis of mesenchymal stem cells isolated from bone marrow (BM), adipose tissue, synovial membrane and umbilical vein wall. We confirmed that BM and adipose tissue derived cells were very similar, which argue for their interchangeable use for cell therapy. We also compared human mesenchymal to embryonic stem cells and showed that umbilical vein wall stem cells, a neo-natal cell type, were closer to BM cells than to embryonic stem cells. Based on these proteomic data, we could propose a panel of proteins which were the basis for the definition of a mesenchymal stem cell proteomic signature.
Databáze: OpenAIRE
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