Biodistribution, Cardiac and Neurobehavioral Assessments, and Neurotransmitter Quantification in Juvenile Rats following Oral Administration of Aluminum Oxide Nanoparticles
| Autor: | Susan Sumner, Maria Moreno Caffaro, Shyam Aravamudhan, Scott L. Watson, Purvi R. Patel, Timothy R. Fennell, Ninell P. Mortensen, Timothy W. Lefever, Rodney W. Snyder, Stephanie A. Montgomery |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
medicine.medical_specialty Biodistribution Administration Oral Metal Nanoparticles Spleen Ileum 010501 environmental sciences Motor Activity Toxicology 01 natural sciences Article Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Electrocardiography Oral administration Internal medicine Aluminum Oxide Medicine Animals Tissue Distribution 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences Neurotransmitter Agents business.industry Homovanillic acid Brain Heart Rats medicine.anatomical_structure Endocrinology chemistry Rotarod Performance Test Toxicity Histopathology Female Lymph business |
| Zdroj: | J Appl Toxicol |
| Popis: | Little is known about the uptake, biodistribution, and biological responses of nanoparticles (NPs) and their toxicity in developing animals. Here, male and female juvenile Sprague-Dawley rats received four consecutive daily doses of 10 mg/kg Al2 O3 NP (diameter: 24 nm [transmission electron microscope], hydrodynamic diameter: 148 nm) or vehicle control (water) by gavage between postnatal days (PNDs) 17-20. Basic neurobehavioral and cardiac assessments were performed on PND 20. Animals were sacrificed on PND 21, and selected tissues were collected, weighed, and processed for histopathology or neurotransmitter analysis. The biodistribution of Al2 O3 NP in tissue sections of the intestine, liver, spleen, kidney, and lymph nodes were evaluated using enhanced dark-field microscopy (EDM) and hyperspectral imaging (HSI). Liver-to-body weight ratio was significantly increased for male pups administered Al2 O3 NP compared with control. HSI suggested that Al2 O3 NP was more abundant in the duodenum and ileum tissue of the female pups compared with the male pups, whereas the abundance of NP was similar for males and females in the other tissues. The abundance of NP was higher in the liver compared with spleen, lymph nodes, and kidney. Homovanillic acid and norepinephrine concentrations in brain were significantly decreased following Al2 O3 NP administration in female and male pups, whereas 5-hydroxyindoleacetic acid was significantly increased in male pups. EDM/HSI indicates intestinal uptake of Al2 O3 NP following oral administration. Al2 O3 NP altered neurotransmitter/metabolite concentrations in juvenile rats' brain tissues. Together, these data suggest that orally administered Al2 O3 NP interferes with the brain biochemistry in both female and male pups. |
| Databáze: | OpenAIRE |
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