The pan-ErbB receptor tyrosine kinase inhibitor canertinib promotes apoptosis of malignant melanoma in vitro and displays anti-tumor activity in vivo
| Autor: | Henrik Gréen, Thomas M. Walz, Anna-Lotta Hallbeck, Olle Stål, Avni Abdiu, Emelie Severinsson, Cecilia Trinks |
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| Rok vydání: | 2011 |
| Předmět: |
STAT3 Transcription Factor
Receptor ErbB-3 medicine.drug_class Receptor ErbB-2 Morpholines Biophysics Mice Nude Antineoplastic Agents Apoptosis Biology Biochemistry Tyrosine-kinase inhibitor chemistry.chemical_compound Mice ErbB medicine Animals Humans Phosphorylation Molecular Biology Protein kinase B Melanoma Protein Kinase Inhibitors Cell Proliferation Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 AXL receptor tyrosine kinase Canertinib Cell growth Cell Biology Protein-Tyrosine Kinases Molecular biology chemistry Cancer research biology.protein Female Tyrosine kinase Proto-Oncogene Proteins c-akt Platelet-derived growth factor receptor |
| Zdroj: | Biochemical and biophysical research communications. 414(3) |
| ISSN: | 1090-2104 |
| Popis: | The ErbB receptor family has been suggested to constitute a therapeutic target for tumor-specific treatment of malignant melanoma. Here we investigate the effect of the pan-ErbB tyrosine kinase inhibitor canertinib on cell growth and survival in human melanoma cells in vitro and in vivo. Canertinib significantly inhibited growth of cultured melanoma cells, RaH3 and RaH5, in a dose-dependent manner as determined by cell counting. Half-maximum growth inhibitory dose (IC(50)) was approximately 0.8 μM and by 5 μM both cell lines were completely growth-arrested within 72 h of treatment. Incubation of exponentially growing RaH3 and RaH5 with 1 μM canertinib accumulated the cells in the G(1)-phase of the cell cycle within 24h of treatment without induction of apoptosis as determined by flow cytometry. Immunoblot analysis showed that 1 μM canertinib inhibited ErbB1-3 receptor phosphorylation with a concomitant decrease of Akt-, Erk1/2- and Stat3 activity in both cell lines. In contrast to the cytostatic effect observed at doses ≤ 5μM canertinib, higher concentrations induced apoptosis as demonstrated by the Annexin V method and Western blot analysis of PARP cleavage. Furthermore, canertinib significantly inhibited growth of RaH3 and RaH5 melanoma xenografts in nude mice. Pharmacological targeting of the ErbB receptors may prove successful in the treatment of patients with metastatic melanoma. |
| Databáze: | OpenAIRE |
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