Characterization of Hen’s Egg White To Use It as a Novel Platform To Culture Three-Dimensional Multicellular Tumor Spheroids
| Autor: | Ramasamy Thirumurugan, Arunachalam Ramkumar, Anbazhagan Murugadas, Sellathamby Shanmugaapriya, Mohammad Abdulkader Akbarsha, Perumalsamy Balaji |
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| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | ACS Omega, Vol 5, Iss 31, Pp 19760-19770 (2020) ACS Omega |
| ISSN: | 2470-1343 |
| DOI: | 10.1021/acsomega.0c02508 |
| Popis: | We are standardizing protocols to develop egg white (EW) as a cost-effective platform for culture of three-dimensional (3-D) multicellular tumor spheroids for application in understanding tumor microenvironments and drug screening. In this article, we describe several physical and physiological characteristics of EW to use it as 3-D cell culture platform. Field emission scanning electron microscopy revealed the presence of different microstructures. Hydrodynamic size distribution data indicated nano- and micron-sized particles. Rheological measurements revealed the viscosity and viscoelastic behavior appropriate for maintaining cell viability and supporting 3-D cell growth under high-sheer conditions. It was found that thereis no autofluorescence, a requirement for imparting transparency and for microscopic observations of the spheroids. The EW facilitated the development of 3-D tumor spheroids, with an emphasis of difference in cell proliferation and intercellular cytoskeletal organization between two-dimensional and 3-D spheroid cultures. Put together, EW proves to be a cost-affordable and simple platform for 3-D culture of tumor spheroids. |
| Databáze: | OpenAIRE |
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