Three-Dimensional Telomeric Analysis of Isolated Circulating Tumor Cells (CTCs) Defines CTC Subpopulations
| Autor: | Mark Chu Xu, Janine Wechsler, Darrel Drachenberg, Julius Adebayo Awe, Yvon E. Cayre, Jeff Saranchuk, Sabine Mai, Naoual Benali-Furet |
|---|---|
| Přispěvatelé: | Sahlgrenska Academy at University of Gothenburg [Göteborg], University of Skövde [Sweden], University of Manitoba [Winnipeg], ScreenCell [Sarcelles], Département de pathologie [Mondor], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Henri Mondor-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), AP-HP Hôpital universitaire Robert-Debré [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Pierre et Marie Curie - Paris 6 (UPMC) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Cancer Research
Lung cancer cell line [SDV.CAN]Life Sciences [q-bio]/Cancer Filtration technique Bioinformatics lcsh:RC254-282 03 medical and health sciences 0302 clinical medicine Circulating tumor cell Prostate Chromosome instability Tumor stage medicine 030304 developmental biology 0303 health sciences business.industry Melanoma lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease 3. Good health Telomere medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Cancer research business Research Article |
| Zdroj: | Translational Oncology Translational Oncology, 2013, 6 (1), pp.51-65. ⟨10.1593/tlo.12361⟩ Translational Oncology, Elsevier, 2013, 6 (1), pp.51-65. ⟨10.1593/tlo.12361⟩ Translational Oncology, Vol 6, Iss 1, Pp 51IN4-65 (2013) |
| ISSN: | 1944-7124 1936-5233 |
| DOI: | 10.1593/tlo.12361⟩ |
| Popis: | International audience; Circulating tumor cells (CTCs) have been identified with the potential to serve as suitable biomarkers for tumor stage and progression, but the availability of effective isolation technique(s) coupled with detailed molecular characterization have been the challenges encountered in making CTCs clinically relevant. For the first time, we combined isolation of CTCs using the ScreenCell filtration technique with quantitative analysis of CTC telomeres by TeloView. This resulted in the identification and molecular characterization of different subpopulations of CTCs in the same patient. Three-dimensional (3D) telomeric analysis was carried out on isolated CTCs of 19 patients that consisted of four different tumor types, namely, prostate, colon, breast, melanoma, and one lung cancer cell line. With telomeric analysis of the filter-isolated CTCs, the level of chromosomal instability (CIN) of the CTCs can be determined. Our study shows that subpopulations of CTCs can be identified on the basis of their 3D telomeric properties. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|