Identification and Characterization of an RTX Toxin–Like Gene and Its Operon from Avibacterium paragallinarum
| Autor: | Yen-Chiang Pan, Jui-Hung Shien, Pin-Chun Shen, Chun-Chi Liu, Duen-Huey Tan, Poa-Chun Chang, Yi-Siang He |
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| Rok vydání: | 2012 |
| Předmět: |
Operon
Bacterial Toxins Molecular Sequence Data medicine.disease_cause Polymerase Chain Reaction law.invention Microbiology Bacterial Proteins Food Animals law medicine Amino Acid Sequence Escherichia coli Gene Polymerase chain reaction General Immunology and Microbiology biology Pasteurellaceae RTX toxin Building and Construction Gene Expression Regulation Bacterial biology.organism_classification Proprotein convertase Molecular biology Recombinant Proteins Recombinant DNA Animal Science and Zoology Genome Bacterial |
| Zdroj: | Avian Diseases. 56:537-544 |
| ISSN: | 1938-4351 0005-2086 |
| Popis: | Avibacterium paragallinarum is the causative agent of infectious coryza, an important respiratory disease of chickens. Whole-genome sequencing analysis showed that A. paragallinarum strain H18 contains an RTX toxin-like operon with strong similarity to the RTX operons of other members of the Pasteurellaceae. Four genes, designated avxIC, avxIA, avxIB, and avxID, were found in this operon. The avxIA gene encodes the structural RTX toxin-like protein, which has a predicted molecular mass of about 250 kDa. The AvxIA protein contains a peptidase S8 domain and a proprotein convertase P-domain, neither of which has been found in other RTX toxins. Recombinant AvxIA proteins expressed in Escherichia coli showed neither hemolytic nor cytotoxic activity. Polymerase chain reaction and sequencing analysis revealed that the avxIA gene was present in all strains and field isolates of A. paragallinarum examined in this study. Sera collected from chickens exposed to A. paragallinarum exhibited strong reactivity to the AvxIA protein, which suggests that AvxIA is immunogenic. This is the first report of the identification of an RTX toxin-like operon from A. paragallinarum. The gene products of this operon may be related to disease pathogenesis and potentially represent a useful vaccine target of A. paragallinarum. |
| Databáze: | OpenAIRE |
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