Structural homo- and heterosynaptic plasticity in mature and adult newborn rat hippocampal granule cells
| Autor: | Tijana Radic, Marcel Beining, Peter Jedlicka, Hermann Cuntz, Tassilo Jungenitz, Thomas Deller, Stephan W. Schwarzacher |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Dendritic Spines Long-Term Potentiation Models Neurological Heterosynaptic plasticity Stimulation Biology Hippocampal formation Cytoplasmic Granules Hippocampus Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine medicine Animals Cells Cultured Neurons Neuronal Plasticity Multidisciplinary Neurogenesis Long-term potentiation Perforant path Electric Stimulation Rats 030104 developmental biology medicine.anatomical_structure Animals Newborn PNAS Plus Synapses Synaptic plasticity NMDA receptor Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Adult newborn hippocampal granule cells (abGCs) contribute to spatial learning and memory. abGCs are thought to play a specific role in pattern separation, distinct from developmentally born mature GCs (mGCs). Here we examine at which exact cell age abGCs are synaptically integrated into the adult network and which forms of synaptic plasticity are expressed in abGCs and mGCs. We used virus-mediated labeling of abGCs and mGCs to analyze changes in spine morphology as an indicator of plasticity in rats in vivo. High-frequency stimulation of the medial perforant path induced long-term potentiation in the middle molecular layer (MML) and long-term depression in the nonstimulated outer molecular layer (OML). This stimulation protocol elicited NMDA receptor-dependent homosynaptic spine enlargement in the MML and heterosynaptic spine shrinkage in the inner molecular layer and OML. Both processes were concurrently present on individual dendritic trees of abGCs and mGCs. Spine shrinkage counteracted spine enlargement and thus could play a homeostatic role, normalizing synaptic weights. Structural homosynaptic spine plasticity had a clear onset, appearing in abGCs by 28 d postinjection (dpi), followed by heterosynaptic spine plasticity at 35 dpi, and at 77 dpi was equally as present in mature abGCs as in mGCs. From 35 dpi on, about 60% of abGCs and mGCs showed significant homo- and heterosynaptic plasticity on the single-cell level. This demonstration of structural homo- and heterosynaptic plasticity in abGCs and mGCs defines the time course of the appearance of synaptic plasticity and integration for abGCs. |
| Databáze: | OpenAIRE |
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