Dynamics of dual-fluorescent polymersomes with durable integrity in living cancer cells and zebrafish embryos
| Autor: | Sven H. C. Askes, Sylvestre Bonnet, Michael S. Meijer, Nelli Bossert, Alexander Kros, Doris Heinrich, Roxanne E. Kieltyka, Victorio Saez Talens, Jeroen Bussmann |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Embryo
Nonmammalian Polymers Biophysics Bioengineering Polyenes 02 engineering and technology 010402 general chemistry Endocytosis 01 natural sciences Polyethylene Glycols Flow cytometry Biomaterials Drug Delivery Systems In vivo medicine Animals Humans Zebrafish Fluorescent Dyes medicine.diagnostic_test Chemistry Vesicle Biological Transport Adenocarcinoma Bronchiolo-Alveolar 021001 nanoscience & nanotechnology In vitro 0104 chemical sciences Cell biology A549 Cells Mechanics of Materials Cancer cell Drug delivery Polymersome Ceramics and Composites Nanoparticles 0210 nano-technology |
| Zdroj: | Biomaterials Biomaterials, 168, 54-63 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2018.03.037 |
| Popis: | The long-term fate of biomedical nanoparticles after endocytosis is often only sparsely addressed in vitro and in vivo, while this is a crucial parameter to conclude on their utility. In this study, dual-fluorescent polyisobutylene-polyethylene glycol (PiB-PEG) polymersomes were studied for several days in vitro and in vivo. In order to optically track the vesicles' integrity, one fluorescent probe was located in the membrane and the other in the aqueous interior compartment. These non-toxic nanovesicles were quickly endocytosed in living A549 lung carcinoma cells but unusually slowly transported to perinuclear lysosomal compartments, where they remained intact and luminescent for at least 90 h without being exocytosed. Fluorescence-assisted flow cytometry indicated that after endocytosis, the nanovesicles were eventually degraded within 7–11 days. In zebrafish embryos, the polymersomes caused no lethality and were quickly taken up by the endothelial cells, where they remained fully intact for as long as 96 h post-injection. This work represents a novel case-study of the remarkable potential of PiB-PEG polymersomes as an in vivo bio-imaging and slow drug delivery platform. |
| Databáze: | OpenAIRE |
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