Identification of Myopia-Related Marker Proteins in Tilapia Retinal, RPE, and Choroidal Tissue following Induced Form Deprivation

Autor:	Thomas D. Singer, Brendan J. McConkey, Wei Shen, Rasmus Jostrup, Jacob G. Sivak, Jeffrey T A Burrows
Rok vydání:	2009
Předmět:	Fish Proteins Proteomics food.ingredient genetic structures Molecular Sequence Data Down-Regulation Retinal Pigment Epithelium Biology Retina Cellular and Molecular Neuroscience chemistry.chemical_compound food Tandem Mass Spectrometry Myopia medicine Animals Electrophoresis Gel Two-Dimensional Amino Acid Sequence Annexin A5 Cloning Molecular Eye Proteins Peptide sequence Gelsolin Retinal pigment epithelium Base Sequence Sequence Homology Amino Acid Choroid Tilapia Retinal Cichlids Anatomy biology.organism_classification Molecular biology eye diseases Sensory Systems Form Perception Disease Models Animal Ophthalmology Oreochromis medicine.anatomical_structure chemistry sense organs Sensory Deprivation Biomarkers Chaperonin Containing TCP-1
Zdroj:	Current Eye Research. 34:966-975
ISSN:	1460-2202 0271-3683
Popis:	Experimentally induced myopia is characterized by axial elongation of the eye. The molecular pathways leading to this condition are largely unknown, even though many candidate proteins have been proposed to be involved in this process. This study has identified proteins that were differentially expressed in myopic and control combined retina, retinal pigment epithelium (RPE), and choroidal tissue in tilapia (Oreochromis niloticus).Form deprivation was used to induce myopia in tilapia (n = 3). In this initial study on tilapia retina, RPE and choroid, 2-D differential in gel electrophoresis (DIGE) and mass spectrometry were used to identify differentially expressed proteins. Homology-based gene cloning was used to obtain full sequence data for one of the identified proteins.A total of 18 protein spots separated by 2-D electrophoresis exhibited statistically significant differences in expression between the myopic and contralateral control combined retinal, RPE, and choroidal tissue. Three proteins were identified at a significance level of p0.05, as annexin A5 (down-regulated 47%), Gelsolin (down-regulated 27%), and TCP-1 (CCT) (down-regulated 54%). DNA sequencing of tilapia annexin A5 shows an amino acid sequence identity of 84.5% with the homologous Japanese ricefish annexin max2.A proteomics approach has been used to identify differentially expressed proteins in form-deprived combined retinal, RPE, and choroidal tissue from myopic versus normal eyes. The identified proteins may be components of pathways involved in myopia pathogenesis.
Databáze:	OpenAIRE
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