Engineered HA hydrogel for stem cell transplantation in the brain: Biocompatibility data using a design of experiment approach
| Autor: | Andrew R. Berg, Jessica K Cinkornpumin, William E. Lowry, Tatiana Segura, S. Thomas Carmichael, Pouria Moshayedi, Irene L. Llorente, Lina R. Nih |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment Cell 02 engineering and technology Regenerative Medicine Ischemia Stem Cell Research - Nonembryonic - Human DOE IKVAV BMP-4 Induced pluripotent stem cell Hyaluronan Brain derived-neurotrophic factor Multidisciplinary Design of experiment RGD Stem Cell Research - Induced Pluripotent Stem Cell - Human Chemistry Stem cell transplantation Brain 021001 nanoscience & nanotechnology Neural stem cell Cell biology Stroke medicine.anatomical_structure lcsh:R858-859.7 Biocompatibility Stem Cell Research - Nonembryonic - Non-Human Development of treatments and therapeutic interventions Stem cell 0210 nano-technology NPC Biotechnology medicine.medical_specialty Bone-morphogenic protein-4 Hyaluronic acid 1.1 Normal biological development and functioning Bioengineering Cell fate determination lcsh:Computer applications to medicine. Medical informatics Brain repair 03 medical and health sciences Underpinning research medicine Progenitor cell YIGSR Stem Cell Research - Embryonic - Human lcsh:Science (General) Data Article Transplantation Toxicity Stem Cell Research - Induced Pluripotent Stem Cell 5.2 Cellular and gene therapies Heparin Growth factor Neurosciences Stem Cell Research Surgery Brain Disorders Hydrogel 030104 developmental biology BDNF Astrocytic scar lcsh:Q1-390 |
| Zdroj: | Data in Brief, Vol 10, Iss C, Pp 202-209 (2017) Data in Brief |
| Popis: | This article presents data related to the research article “Systematic optimization of an engineered hydrogel allows for selective control of human neural stem cell survival and differentiation after transplantation in the stroke brain” (P. Moshayedi, L.R. Nih, I.L. Llorente, A.R. Berg, J. Cinkornpumin, W.E. Lowry et al., 2016) [1] and focuses on the biocompatibility aspects of the hydrogel, including its stiffness and the inflammatory response of the transplanted organ. We have developed an injectable hyaluronic acid (HA)-based hydrogel for stem cell culture and transplantation, to promote brain tissue repair after stroke. This 3D biomaterial was engineered to bind bioactive signals such as adhesive motifs, as well as releasing growth factors while supporting cell growth and tissue infiltration. We used a Design of Experiment approach to create a complex matrix environment in vitro by keeping the hydrogel platform and cell type constant across conditions while systematically varying peptide motifs and growth factors. The optimized HA hydrogel promoted survival of encapsulated human induced pluripotent stem cell derived-neural progenitor cells (iPS-NPCs) after transplantation into the stroke cavity and differentially tuned transplanted cell fate through the promotion of glial, neuronal or immature/progenitor states. The highlights of this article include: (1) Data of cell and bioactive signals addition on the hydrogel mechanical properties and growth factor diffusion, (2) the use of a design of Experiment (DOE) approach (M.W. 2 Weible and T. Chan-Ling, 2007) [2] to select multi-factorial experimental conditions, and (3) Inflammatory response and cell survival after transplantation. |
| Databáze: | OpenAIRE |
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