Anticancer Effects of Phenoxazine Derivatives Revealed by Inhibition of Cell Growth and Viability, Disregulation of Cell Cycle, and Apoptosis Induction in HTLV-1–Positive Leukemia Cells
| Autor: | Kunihiko Kurosaki, Akio Tomoda, Mari Hanami, Hoshimi Aoyagi, Naoko Miyano-Kurosaki, Takahiko Endo, Jun Yasumoto, Kou Ikegami |
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| Rok vydání: | 2009 |
| Předmět: |
Cell Survival
viruses T-cell leukemia Antineoplastic Agents Apoptosis Biology Giant Cells Necrosis Cell Line Tumor Oxazines medicine Animals Humans Cytotoxic T cell RNA Neoplasm Viability assay Cell Proliferation Pharmacology Human T-lymphotropic virus 1 Syncytium Caspase 3 Reverse Transcriptase Polymerase Chain Reaction Cell growth Cell Cycle lcsh:RM1-950 digestive oral and skin physiology Cell cycle medicine.disease HTLV-I Infections Rats Cell biology Leukemia lcsh:Therapeutics. Pharmacology Cell culture Cancer research Molecular Medicine Indicators and Reagents |
| Zdroj: | Journal of Pharmacological Sciences, Vol 110, Iss 1, Pp 87-97 (2009) |
| ISSN: | 1347-8648 1347-8613 |
| Popis: | Adult T-cell leukemia (ATL) is a malignant tumor of human CD4+ T cells infected with a human retrovirus, T lymphotropic virus type-1 (HTLV-1). The aim of the present study was to investigate the apoptotic effects of phenoxazines, 2-amino-4,4α-dihydro-4α,7-dimethyl-3H-phenoxazine-3-one (Phx-1), 3-amino-1,4α-dihydro-4α,8-dimethyl-2H-phenoxazine-2-one (Phx-2), and 2-aminophenoxazine-3-one (Phx-3) on a T cell leukemia cell line from ATL patients, MT-1 cells; HTLV-1 transformed T-cell lines, HUT-102 cells and MT-2 cells; and an HTLV-1–negative rat sarcoma cell line, XC cells. Among these phenoxazines, Phx-3 at concentrations of less than 10 μg/ml extensively inhibited growth and cell viability; arrested cell cycles at sub G0/G1 phase; and augmented apoptosis of MT-1, HUT-102, and MT-2 cells. However, these phenoxazines did not affect the cell viability of an HTLV-1–negative rat sarcoma cell line, XC cells, and phytohemaggutinin-activated human peripheral blood mononuclear cells, although they markedly inhibited the growth of these cells. The transmission of HTLV-1 from HTLV-1– positive cells (MT-2 cells) to HTLV-1–negative cells (XC cells) was considered to be prevented by Phx-1, Phx-2, or Phx-3 because the syncytium formation between these cells was inhibited markedly in the presence of these phenoxazines. The present results suggest that Phx-1, Phx-2, and, in particular, Phx-3 may be useful as therapeutic agents against ATL, which is extremely refractory to current therapies. Keywords:: adult T-cell leukemia, HTLV-1, phenoxazine, apoptosis, syncytium formation |
| Databáze: | OpenAIRE |
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