Asymmetrically functional surface properties on biocompatible phospholipid polymer membrane for bioartificial kidney
| Autor: | Tomohiro Konno, Hideto Ueda, Junji Watanabe, Kazuhiko Ishihara, Madoka Takai, Akira Saito |
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| Rok vydání: | 2006 |
| Předmět: |
Blood Platelets
Materials science Polymers Surface Properties Biomedical Engineering Biocompatible Materials Biomaterials chemistry.chemical_compound Adsorption Materials Testing Polymer chemistry Animals Humans Sulfones Polysulfone Phase inversion (chemistry) Cells Cultured Phospholipids chemistry.chemical_classification Bioartificial Organs Metals and Alloys Epithelial Cells Membranes Artificial Polymer Membrane chemistry Ceramics and Composites Biophysics Polymer blend beta 2-Microglobulin Layer (electronics) Protein adsorption |
| Zdroj: | Journal of Biomedical Materials Research Part A. :19-27 |
| ISSN: | 1552-4965 1549-3296 |
| DOI: | 10.1002/jbm.a.30606 |
| Popis: | To obtain a bioartificial kidney composed of a porous polymer membrane and renal cells, a polysulfone (PSf) membrane (PSM) blended with 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer was prepared. The PSM flat membrane with a porous structure could be prepared from the polymer blend containing 1 wt % of the MPC polymer in PSf by the phase inversion technique in a dry-wet process. Asymmetrical surface properties were observed on both sides of the membrane surfaces. That is, the sponge layer formed at the substrate-contacting surface of the membrane had 10-20 microm pores, but the pores in the micrometer range could not be observed for a skin layer formed at the air-contacting surface of the membrane. At the sponge layer surface, the MPC unit composition was 7 times larger than that at the skin layer surface. The amount of proteins adsorbed on the surface corresponded to the MPC unit composition. On the skin layer, a small amount of adsorbed proteins and platelet adhesion could be suppressed compared with those on the sponge layer. However, the skin layer had a moderate protein adsorption, so it showed a sufficient cytocompatibility to enable renal tubule epithelial cells to adhere and proliferate in the membrane. Thus, it functioned well as a renal tubule. Therefore, because of both its hemocompatibility and cytocompatibility, we could conclude that the PSM membrane is useful for as a renal tubule device for a bioartificial kidney. |
| Databáze: | OpenAIRE |
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