Buggy Creek Virus (Togaviridae:Alphavirus) Upregulates Expression of Pattern Recognition Receptors and Interferons in House Sparrows (Passer domesticus)
| Autor: | Virginia A. Barak, Ellecia L. Rainwater, Ashley M. Altrichter, Carol A. Fassbinder-Orth |
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| Rok vydání: | 2014 |
| Předmět: |
Gene Expression Regulation
Viral viruses Alpha interferon Viremia Alphavirus Microbiology Virus Interferon-gamma Random Allocation Interferon Virology medicine Animals Viral shedding Alphavirus infection Muscle Skeletal biology Alphavirus Infections Bird Diseases Brain Interferon-alpha virus diseases Heart Original Articles biology.organism_classification medicine.disease Up-Regulation Virus Shedding Infectious Diseases Receptors Pattern Recognition Togaviridae RNA Viral Interferons Sparrows Spleen medicine.drug |
| Zdroj: | Vector-Borne and Zoonotic Diseases. 14:439-446 |
| ISSN: | 1557-7759 1530-3667 |
| DOI: | 10.1089/vbz.2013.1531 |
| Popis: | Birds serve as reservoirs for at least 10 arthropod-borne viruses, yet specific immune responses of birds to arboviral infections are relatively unknown. Here, adult House Sparrows were inoculated with an arboviral alphavirus, Buggy Creek virus (BCRV), or saline, and euthanized between 1 and 3 days postinoculation. Virological dynamics and gene expression dynamics were investigated. Birds did not develop viremia postinoculation, but cytopathic virus was found in the skeletal muscle and spleen of birds 1 and 3 days postinoculation (DPI). Viral RNA was detected in the blood of BCRV-infected birds 1 and 2 DPI, in oral swabs 1–3 DPI, and in brain, heart, skeletal muscle, and spleen 1–3 DPI. Multiple genes were significantly upregulated following BCRV infection, including pattern recognition receptors (TLR7, TLR15, RIG-1), type I interferon (IFN-α), and type II interferon (IFN-γ). This is the first study to report avian immunological gene expression profiles following an arboviral infection. |
| Databáze: | OpenAIRE |
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