Bioprinting of Human Liver-derived Epithelial Organoids for Toxicity Studies
| Autor: | Bouwmeester, Manon C, Bernal, Paulina Nunez, Oosterhoff, Loes A, van Wolferen, Monique, Lehmann, Vivian, Vermaas, Monique, Buchholz, Maj-Britt, Peiffer, Quentin, Malda, Jos, van der Laan, Luc J W, Kramer, Nynke I, Schneeberger, Kerstin, Levato, Riccardo, Spee, Bart, Interne geneeskunde GD, dCSCA RMSC-1, Equine Musculoskeletal Biology, dES RMSC, dIRAS RA-1, CS_STEM |
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| Přispěvatelé: | Interne geneeskunde GD, dCSCA RMSC-1, Equine Musculoskeletal Biology, dES RMSC, dIRAS RA-1, CS_STEM, Surgery |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Polymers and Plastics
Cell Cell Culture Techniques in vitro modeling Bioengineering 02 engineering and technology Toxicology drug induced liver injury Biomaterials 03 medical and health sciences SDG 3 - Good Health and Well-being medicine Organoid Materials Chemistry Humans Viability assay Cells Cultured Toxicologie organoids 030304 developmental biology Liver injury 0303 health sciences Human liver Tissue Engineering Chemistry Bioprinting 021001 nanoscience & nanotechnology medicine.disease In vitro Cell biology medicine.anatomical_structure Liver Toxicity Printing Three-Dimensional extrusion-based bioprinting 0210 nano-technology Biofabrication Biotechnology |
| Zdroj: | Macromolecular Bioscience, 21(12), 1. Wiley-VCH Verlag Macromolecular Bioscience 21 (2021) 12 Macromolecular Bioscience Macromolecular Bioscience, 21(12) Macromolecular Bioscience, 21(12):2100327. Wiley-VCH |
| ISSN: | 1616-5187 |
| Popis: | There is a need for long-lived hepatic in vitro models to better predict drug induced liver injury (DILI). Human liver-derived epithelial organoids are a promising cell source for advanced in vitro models. Here, organoid technology is combined with biofabrication techniques, which holds great potential for the design of in vitro models with complex and customizable architectures. Here, porous constructs with human hepatocyte-like cells derived from organoids are generated using extrusion-based printing technology. Cell viability of bioprinted organoids remains stable for up to ten days (88–107% cell viability compared to the day of printing). The expression of hepatic markers, transporters, and phase I enzymes increased compared to undifferentiated controls, and is comparable to non-printed controls. Exposure to acetaminophen, a well-known hepatotoxic compound, decreases cell viability of bioprinted liver organoids to 21–51% (p < 0.05) compared to the start of exposure, and elevated levels of damage marker miR-122 are observed in the culture medium, indicating the potential use of the bioprinted constructs for toxicity testing. In conclusion, human liver-derived epithelial organoids can be combined with a biofabrication approach, thereby paving the way to create perfusable, complex constructs which can be used as toxicology- and disease-models. |
| Databáze: | OpenAIRE |
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