Nuclear transferred embryonic stem cells for analysis of B1 B-lymphocyte development
| Autor: | Osami Kanagawa, Mitsuyo Takase, Sidonia Fagarasan, Ryuji Iida, Mikako Maruya, Satoshi Nishigami, Atsushi Miyawaki, Teruhiko Wakayama, Asako Sakaue-Sawano |
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| Rok vydání: | 2012 |
| Předmět: |
Nuclear Transfer Techniques
Lymphocyte Cellular differentiation Immunology Receptors Antigen B-Cell Mice Transgenic Cell Line Immunoglobulin kappa-Chains Mice Immunoglobulin lambda-Chains Antigen medicine Animals Immunology and Allergy Cell Lineage Gene Rearrangement B-Lymphocyte Peritoneal Cavity Embryonic Stem Cells B-Lymphocytes Mice Inbred BALB C CD40 biology Cell Differentiation General Medicine Embryonic stem cell Immunity Innate Cell biology Mice Inbred C57BL B-1 cell medicine.anatomical_structure Mice Inbred DBA Cell culture biology.protein Immunoglobulin Heavy Chains Clone (B-cell biology) |
| Zdroj: | International Immunology. 25:145-156 |
| ISSN: | 1460-2377 0953-8178 |
| DOI: | 10.1093/intimm/dxs095 |
| Popis: | The transfer of nuclei of fully differentiated cells into enucleated oocytes is a well-recognized method for the generation of embryonic stem (ES) cells. Here, we demonstrate that nuclear transferred ES (NT-ES) cells can be established with high efficiency using innate-like B lymphocytes as donor cells. We established two mouse lines carrying rearranged immunoglobulin heavy and light chains using NT-ES cells containing nuclei from peritoneal cavity B1 cells. Analysis of B1 clone lines revealed that the B1-cell generation critically depends on the interaction between antigen (possibly self-antigen) and surface immunoglobulin, while the B1-cell maintenance requires the peritoneal environment. The B1-cell expansion takes place in spleen, and is held in check by competitor B2 cells. The results indicate that the NT-ES method could replace the transgenic or knock-in mouse approaches currently used to study the biology of cells that undergo somatic rearrangements of their antigen receptor genes. |
| Databáze: | OpenAIRE |
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