Breviscapine ameliorates cardiac dysfunction and regulates the myocardial Ca2+-cycling proteins in streptozotocin-induced diabetic rats
| Autor: | Guosheng Fu, Junhui Zhu, Bin-quan Zhou, Min Wang, Wenbin Zhang |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
Cardiac function curve medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment Intraperitoneal injection Gene Expression Diabetes Mellitus Experimental Sarcoplasmic Reticulum Calcium-Transporting ATPases Rats Sprague-Dawley Endocrinology Diabetic cardiomyopathy Internal medicine Internal Medicine medicine Animals Humans Protein kinase C Flavonoids Calcium metabolism business.industry Ryanodine receptor Myocardium Calcium-Binding Proteins Intracellular Signaling Peptides and Proteins Heart Ryanodine Receptor Calcium Release Channel General Medicine medicine.disease Streptozotocin Rats Phospholamban cardiovascular system Calcium business Drugs Chinese Herbal medicine.drug |
| Zdroj: | Acta Diabetologica. 47:209-218 |
| ISSN: | 1432-5233 0940-5429 |
| DOI: | 10.1007/s00592-009-0164-x |
| Popis: | To investigate the influence of breviscapine on the cardiac structure and function in diabetic cardiomyopathy rats as well as the expression of protein kinase C (PKC) and Ca(2+)-cycling proteins expression. Diabetes was induced in male Sprague-Dawley rats by a single intraperitoneal injection of streptozotocin and the control rats were injected with saline. After the induction of diabetes for 4 weeks, the animals were divided into different groups: (1) normal rats as control; (2) diabetic rats; (3) diabetic rats with administration of breviscapine (10 or 25 mg kg(-1) day(-2)). After treatment with breviscapine for 6 weeks, the invasive cardiac function and echocardiographic parameters were measured, and heart tissue was obtained for electron microscope study. The expression of protein kinase C (PKC) and calcium handling regulators, such as protein phosphatase inhibitor-1 (PPI-1), phospholamban (PLB) and Ca(2+)-ATPase (SERCA-2), ryanodine receptor (RyR) were detected by western blot or RT-PCR. The activity of SERCA-2 was measured using Ca(2+)-ATPase kit. Diabetic rats showed impaired cardiac structure and function compared with control rats. The expression of PKC, PLB increased significantly, while the PPI-1, SERCA-2 and RyR expression decreased. Treatment with breviscapine could reverse the cardiac dysfunction and structure changes in diabetic cardiomyopathy rats, and decrease the expression of PKC and PLB, as well as increase the expression of PPI-1, SERCA-2 and RyR. The protective effect of breviscapine was dose related. This study showed that breviscapine could regulate the expression of PKC, PPI-1, PLB and SERCA-2 and have protective effect on diabetic cardiomyopathy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink