The Adipocyte as an Important Target Cell for Trypanosoma cruzi Infection
| Autor: | Nagajyothi, Stephen M. Factor, Shankar Mukherjee, David Jayabalan, Cecilia J. de Almeida, Linda A. Jelicks, Aisha Cordero, Dazhi Zhao, Vicki L. Braunstein, Louis M. Weiss, Michael P. Lisanti, Philipp E. Scherer, Herbert B. Tanowitz, Ying Lin, Terry P. Combs, Shira Landskroner-Eiger, William Schubert |
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| Rok vydání: | 2005 |
| Předmět: |
medicine.medical_specialty
Trypanosoma cruzi Blotting Western Adipokine Adipose tissue Parasitemia Biochemistry Pathogenesis Mice chemistry.chemical_compound Trypanosomiasis 3T3-L1 Cells Internal medicine Adipocyte Lipid droplet Adipocytes medicine Animals Molecular Biology DNA Primers Base Sequence biology Cell Biology medicine.disease biology.organism_classification Immunohistochemistry Hypoglycemia Chronic infection Endocrinology chemistry Immunology Microscopy Electron Scanning |
| Zdroj: | Journal of Biological Chemistry. 280:24085-24094 |
| ISSN: | 0021-9258 |
| Popis: | Adipose tissue plays an active role in normal metabolic homeostasis as well as in the development of human disease. Beyond its obvious role as a depot for triglycerides, adipose tissue controls energy expenditure through secretion of several factors. Little attention has been given to the role of adipocytes in the pathogenesis of Chagas disease and the associated metabolic alterations. Our previous studies have indicated that hyperglycemia significantly increases parasitemia and mortality in mice infected with Trypanosoma cruzi. We determined the consequences of adipocyte infection in vitro and in vivo. Cultured 3T3-L1 adipocytes can be infected with high efficiency. Electron micrographs of infected cells revealed a large number of intracellular parasites that cluster around lipid droplets. Furthermore, infected adipocytes exhibited changes in expression levels of a number of different adipocyte-specific or adipocyte-enriched proteins. The adipocyte is therefore an important target cell during acute Chagas disease. Infection of adipocytes by T. cruzi profoundly influences the pattern of adipokines. During chronic infection, adipocytes may represent an important long-term reservoir for parasites from which relapse of infection can occur. We have demonstrated that acute infection has a unique metabolic profile with a high degree of local inflammation in adipose tissue, hypoadiponectinemia, hypoglycemia, and hypoinsulinemia but with relatively normal glucose disposal during an oral glucose tolerance test. |
| Databáze: | OpenAIRE |
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