Time-Resolved Loading of Monomers into Bilayers with Different Curvature
| Autor: | Eugene Pinkhassik, S. C. Schaub, Andrew G. Richter, Sergey A. Dergunov |
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| Rok vydání: | 2009 |
| Předmět: |
Liposome
Chemistry Bilayer Lipid Bilayers Analytical chemistry Benzene Surfaces and Interfaces Lipid bilayer mechanics Condensed Matter Physics Article Kinetics Chemical engineering Dynamic light scattering Liposomes Amphiphile Electrochemistry General Materials Science Self-assembly Lipid bilayer phase behavior Dimyristoylphosphatidylcholine Lipid bilayer Hydrophobic and Hydrophilic Interactions Chromatography High Pressure Liquid Spectroscopy |
| Zdroj: | Langmuir. 26:6276-6280 |
| ISSN: | 1520-5827 0743-7463 |
| Popis: | Directed assembly of nanostructures within temporary and recyclable self-assembled scaffolds is emerging as an attractive method for the synthesis of nanomaterials with programmed properties. Understanding interactions of building blocks with amphiphilic scaffolds is critical for rational design of new nanostructures and nanodevices. Here we examine loading of hydrophobic monomers into bilayers with different curvatures. Time-resolved loading was studied by high performance liquid chromatography and dynamic light scattering. Despite differences in initial bilayer geometry, loading rates and maximum bilayer capacity are the same for liposomes with radii ranging from 25 to 100 nm. When using divinylbenzene (DVB) and dimyristoylphosphatidylcholine (DMPC), monomer/lipid loading ratio of 1.2 was achieved within 12 hours. While accommodation of a large amount of monomers is likely to be accompanied with significant changes in bilayer structure, all liposomes in this study including those with smallest size and higher bilayer curvature retain encapsulated content and show no evidence of fusion during monomer loading. These results contribute to our understanding of interactions between hydrophobic molecules and lipid bilayers and expand the scope of the directed assembly method. |
| Databáze: | OpenAIRE |
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