Identification of a polypeptide DNA polymerase inhibitor responsible for ribonuclease inhibition of an endogenous DNA polymerase reaction
| Autor: | A.R. Page, D.M. Oman |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
DNA polymerase
DNA polymerase II Biophysics Thymus Gland Biochemistry DNA polymerase delta Micrococcus Ribonucleases Microsomes Animals Trypsin Molecular Biology Polymerase DNA clamp biology Chemistry Cell Biology DNA Molecular biology Reverse transcriptase DNA Nucleotidyltransferases biology.protein DNA Polymerase Inhibitor RNA Lymph Nodes Rabbits DNA polymerase I Peptides |
| Zdroj: | Biochemical and biophysical research communications. 61(4) |
| ISSN: | 0006-291X |
| Popis: | The microsomal fraction from rabbit lymph node and thymus cells contains endogenous DNA polymerase activity that is inhibited by ribonuclease. The template for the endogenous reaction was shown to be DNA and the enzyme has the general characteristics of cellular DNA dependent DNA polymerase. The ribonuclease inhibition of the reaction was found to be due to a polypeptide inhibitor of the DNA polymerase enzyme. This inhibitor is in turn inhibited by RNA and becomes active when RNA is destroyed by ribonuclease or micrococcal nuclease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|