Identification of a sphingosine-sensitive Ca2+ channel in the plasma membrane of Leishmania mexicana
| Autor: | Yael García-Marchan, Claudia Reyes, Graciela L. Uzcanga, Katherine Figarella, Gustavo Benaim |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Calcium Channels
L-Type Leishmania mexicana Molecular Sequence Data Biophysics Biology Pharmacology Ceramides Biochemistry chemistry.chemical_compound Nifedipine Sequence Analysis Protein Sphingosine medicine Humans Amino Acid Sequence Molecular Biology Voltage-dependent calcium channel Calcium channel Cell Membrane Cell Biology 3-Pyridinecarboxylic acid 1 4-dihydro-2 6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)- Methyl ester Calcium Channel Blockers biology.organism_classification Sphingolipid Protein Structure Tertiary Cell biology Calcium Channel Agonists Verapamil chemistry Calcium Homeostasis medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 430:1091-1096 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2012.12.033 |
| Popis: | The disruption of the intracellular Ca(2+) homeostasis of Leishmania mexicana represents a major target for the action of drugs, such as amiodarone and miltefosine. However, little is known about the mechanism of Ca(2+) entry to these cells. Here we show the presence of a Ca(2+) channel in the plasma membrane of these parasites. This channel has many characteristics similar to the human L-type voltage-gated Ca(2+) channel. Thus, Ca(2+) entry is blocked by verapamil, nifedipine and diltiazem while Bay K 8644 opened this channel. However, different to its human counterpart, sphingosine was able to open this channel, while other well known sphingolipids had no effect. This fact could have important pharmacological implications. |
| Databáze: | OpenAIRE |
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