Early preantral follicles of the domestic cat express gonadotropin and sex steroid signaling potential
| Autor: | Shauna Kehoe, Paul R. Johnston, Katarina Jewgenow, Beate C. Braun |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Receptors
Steroid endocrine system 17-Hydroxysteroid Dehydrogenases medicine.drug_class Biology Estradiol Dehydrogenases Andrology Receptors Gonadotropin Cytochrome P-450 Enzyme System Ovarian Follicle medicine Animals Ovarian follicle Gonadal Steroid Hormones PGRMC1 Sequence Analysis RNA Cholesterol side-chain cleavage enzyme Cell Biology General Medicine Sex hormone receptor medicine.anatomical_structure Gene Expression Regulation Reproductive Medicine Sex steroid CYP17A1 Cats Female Folliculogenesis Gonadotropin Gonadotropins Signal Transduction |
| Zdroj: | Biology of Reproduction. 106:95-107 |
| ISSN: | 1529-7268 0006-3363 |
| Popis: | Key biomolecular processes, which regulate primordial ovarian follicle dormancy and early folliculogenesis in mammalian ovaries, are not fully understood. The domestic cat is a useful model to study ovarian folliculogenesis and is the most relevant for developing in vitro growth methods to be implemented in wild felid conservation breeding programs. Previously, RNA-sequencing of primordial (PrF), primary (PF), and secondary follicle (SF) samples from domestic cat implicated ovarian steroidogenesis and steroid reception during follicle development. Here, we aimed to identify which sex steroid biosynthesis and metabolism enzymes, gonadotropin receptors, and sex steroid receptors are present and may be potential regulators. Differential gene expression, functional annotation, and enrichment analyses were employed and protein localization was studied too. Gene transcripts for PGR, PGRMC1, AR (steroid receptors), CYP11A1, CYP17A1, HSD17B1 and HSD17B17 (steroidogenic enzymes), and STS (steroid metabolizing enzyme) were significantly differentially expressed (Q values of ≤0.05). Differential gene expression increased in all transcripts during follicle transitions apart from AR which decreased by the secondary stage. Immunohistochemistry localized FSHR and LHCGR to oocytes at each stage. PGRMC1 immunostaining was strongest in granulosa cells, whereas AR was strongest in oocytes throughout each stage. Protein signals for steroidogenic enzymes were only detectable in SFs. Products of these significantly differentially expressed genes may regulate domestic cat preantral folliculogenesis. In vitro growth could be optimized as all early follicles express gonadotropin and steroid receptors meaning hormone interaction and response may be possible. Protein expression analyses of early SFs supported its potential for producing sex steroids. |
| Databáze: | OpenAIRE |
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