Complete genome sequence of Oryctes rhinoceros nudivirus isolated from the coconut rhinoceros beetle in Solomon Islands
| Autor: | Helen Tsatsia, Gregor J. Devine, Kayvan Etebari, Gordana Rašić, Igor Filipović, Michael J. Furlong |
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| Rok vydání: | 2020 |
| Předmět: |
Cocos
Cancer Research Rhinoceros Genome Viral Genome Open Reading Frames 03 medical and health sciences Virology Animals Nudiviridae ORFS Gene 030304 developmental biology Whole genome sequencing Genetics Scarabaeidae 0303 health sciences Whole Genome Sequencing biology 030306 microbiology Nucleic acid sequence Sequence Analysis DNA biology.organism_classification Coleoptera Infectious Diseases Biological Control Agents Oryctes Female Melanesia |
| Zdroj: | Virus Research. 278:197864 |
| ISSN: | 0168-1702 |
| DOI: | 10.1016/j.virusres.2020.197864 |
| Popis: | Oryctes rhinoceros nudivirus (OrNV) has been an effective biocontrol agent against the insect pest Oryctes rhinoceros (Coleoptera: Scarabaeidae) for decades, but there is evidence that resistance could be evolving in some host populations. We detected OrNV infection in O. rhinoceros from Solomon Islands and used Oxford Nanopore Technologies (ONT) long-read sequencing to determine the full length of the virus genomic sequence isolated from an individual belonging to a mitochondrial lineage (CRB-G) that was previously reported as resistant to OrNV. The complete circular genome of the virus consisted of 125,917 nucleotides, 1.698 bp shorter than the originally-described full genome sequence of Ma07 strain from Malaysia. We found 130 out of 139 previously annotated ORFs (seven contained interrupted/non-coding sequences, two were identified as duplicated versions of the existing genes), as well as a putatively inverted regions containing four genes. These results demonstrate the usefulness of a long-read sequencing technology for resolving potential structural variations when describing new virus isolates. While the Solomon Islands isolate exhibited 99.41 % nucleotide sequence identity with the originally described strain, we found several genes, including a core gene (vlf-1), that contained multiple amino acid insertions and/or deletions as putative polymorphisms of large effect. Our complete annotated genome sequence of a newly found isolate in Solomon Islands provides a valuable resource to help elucidate the mechanisms that compromise the efficacy of OrNV as a biocontrol agent against the coconut rhinoceros beetle. |
| Databáze: | OpenAIRE |
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