Zhengella mangrovi gen. nov., sp. nov., a novel member of family Phyllobacteriaceae isolated from mangrove sediment
| Autor: | Qiliang Lai, Xiaolan Lin, Yu-Qian Li, Yun Tian, Hu Liao |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
DNA Bacterial China Geologic Sediments food.ingredient Ubiquinone Biology medicine.disease_cause Microbiology 03 medical and health sciences food Genus RNA Ribosomal 16S medicine Phyllobacterium Ecology Evolution Behavior and Systematics Phospholipids Phylogeny Base Composition Strain (chemistry) Phylogenetic tree Fatty Acids Aliihoeflea General Medicine Phyllobacteriaceae Sequence Analysis DNA 16S ribosomal RNA rpoB biology.organism_classification Bacterial Typing Techniques 030104 developmental biology Hoeflea Avicennia |
| Zdroj: | International journal of systematic and evolutionary microbiology. 68(9) |
| ISSN: | 1466-5034 |
| Popis: | A Gram-negative strain, designed X9-2-2T, was isolated from mangrove sediment in Yunxiao Mangrove National Nature Reserve, China. Strain X9-2-2T showed less than 96.2 % 16S rRNA gene sequence similarity to type strains of species with validly published names. Phylogenetic analysis based on 16S rRNA gene sequences and rpoB protein sequences revealed that strain X9-2-2T formed a distinct monophyletic clade within the family Phyllobacteriaceae and clustered distantly with the genera Aliihoeflea , Phyllobacterium and Hoeflea . Cells of X9-2-2T were rod-shaped, motile with subpolar or lateral flagella and facultative anaerobic. Optimal growth occurred at 30–37 °C, at pH 7 and in the presence of 2 % NaCl. The DNA G+C content of strain X9-2-2T was 64.9 mol%. The major fatty acids were summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c 56.0 %), iso -C17 : 0 (9.1 %) and C12 : 0 (6.6 %). The predominant respiratory quinone was ubiquinone-10 and the major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine, an unidentified aminolipid and two unidentified polar lipids. According to its morphology, physiology, fatty acid composition and 16S rRNA gene signature nucleotide patterns, strain X9-2-2T represents a novel species of a novel genus in the family Phyllobacteriaceae , for which the name Zhengella mangrovi gen. nov., sp. nov. is proposed. The type strain is X9-2-2T (=MCCC 1K03307T=JCM 32107T). |
| Databáze: | OpenAIRE |
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