Liposomes and MTT cell viability assay: an incompatible affair

Autor:	Alice Floris, Fabrizio Angius
Rok vydání:	2014
Předmět:	Liposome Formazans Cell Survival Tetrazolium Salts General Medicine Biology Toxicology Molecular biology chemistry.chemical_compound Thiazoles chemistry Biochemistry Lipid droplet Liposomes Bioassay Humans MTT assay Biological Assay Viability assay Formazan Cytotoxicity Cell survival HeLa Cells
Zdroj:	Toxicology in vitro : an international journal published in association with BIBRA. 29(2)
ISSN:	1879-3177
Popis:	The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is commonly used to evaluate the cytotoxicity potential of drugs vehicled by liposomes. However, liposome delivering drugs could produce inconsistent values of MTT absorbance. On the basis of previous experiments demonstrating the MTT affinity for lipid droplets, this paper aims to show that empty-liposomes interfere, per se, on MTT assay due to its lipidic nature. This brings into question the use of MTT testing cytotoxicity when liposomes are involved in delivering drugs.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ed7a4ec50d57c089a3dd17600312560 https://pubmed.ncbi.nlm.nih.gov/25481524 Zobrazit plný text záznamu Full Text from ScienceDirect