Automated Electron Tomography of the Septal Pore Cap in Rhizoctonia solani

Autor: Bruno M. Humbel, Wally H. Müller, Ulrike Ziese, Teun Boekhout, Roy Christiaan Montijn, Abraham J. Koster, Arie J. Verkleij, A.C. van Aelst, T.P. van der Krift
Přispěvatelé: Centraal Instituut voor Voedingsonderzoek TNO
Rok vydání: 2000
Předmět:
Laboratorium voor Plantencelbiologie
Endoplasmic Reticulum
Dolipore septum
Cryo-scanning electron microscopy
Cell membrane
Automation
Structural Biology
Freezing
Image Processing
Computer-Assisted
Tomography
Diagnostic value
Priority journal
Fungus
High-pressure freezing
Vesicle
Cell ultrastructure
Mitochondria
Cell biology
Laboratory of Plant Cell Biology
Membrane structure
Intercellular Junctions
medicine.anatomical_structure
6-glucan
Electron beam tomography
Channel gating
Filamentous structures
Thanatephorus cucumeris
animal structures
β-1
6-glucan
Electrons
Biology
Septal pore cap
Cell communication
Rhizoctonia
ZIO staining
Organelle
medicine
β-1
Nutrition
Hyphomycetes
Rhizoctonia solani
Basidiomycota
Endoplasmic reticulum
Cell Membrane
Cryoelectron Microscopy
fungi
Botany
Fungi
Intracellular Membranes
Nonhuman
Microscopy
Electron
Electron tomography
Freeze substitution
Microscopy
Electron
Scanning
Ultrastructure
Transmission electron microscopy
Zdroj: Journal of Structural Biology, 1, 131, 10-18
Journal of Structural Biology 131 (2000)
Journal of Structural Biology, 131, 10-18
ISSN: 1047-8477
DOI: 10.1006/jsbi.2000.4243
Popis: Dolipore septa and septal pore caps (SPCs) in filamentous basidiomycetes may play an important role in maintaining the integrity of hypal cells. We have investigated the ultrastructure of the dolipore septum and the SPC in Rhizoctonia solani hyphal cells after high-pressure freezing, freeze substitution, and Spurr embedding. We visualized the SPC with associated cell ultrastructures in three dimensions by automated electron tomography of thick-sectioned cells, followed by 3D tomographic reconstructions. Using these methods we were able to document the passage of mitochondria through the SPC, small tubular membranous structures at the entrance of the septal pore channel, filamentous structures connecting the inner side of the SPC with pore-plugging material, thin filaments anchoring the pore-plugging material with the plasma membrane, small vesicles attached to the plugging material, and tubular endoplasmic reticulum continuous with the base of the SPC. We hypothesize that the SPC, the filamentous structures, the plugging material, and the endoplasmic reticulum act in a coordinated fashion to maintain cellular integrity, intercellular communication, and the transport of solutes and cell organelles in the filamentous fungus R. solani. (C) 2000 Academic Press.
Databáze: OpenAIRE
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