mGlu1 receptor canonical signaling pathway contributes to the opening of the orphan GluD2 receptor
| Autor: | Selma Dadak, Elise Goyet, Nathalie Bouquier, Carole Levenes, Laurent Fagni, Julie Perroy |
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| Přispěvatelé: | Centre Neurosciences intégratives et Cognition (INCC - UMR 8002), Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
MESH: Signal Transduction Indoles Receptors Metabotropic Glutamate Methoxyhydroxyphenylglycol Maleimides Mice 0302 clinical medicine Cerebellum MESH: Animals Estrenes ComputingMilieux_MISCELLANEOUS MESH: Maleimides MESH: Indoles Metabotropic glutamate receptor 5 Metabotropic glutamate receptor 4 Metabotropic glutamate receptor 7 Metabotropic glutamate receptor 6 Cross-talk Glutamate receptors Pyrrolidinones Receptors Glutamate mGlu1 MESH: HEK293 Cells Metabotropic glutamate receptor 1 [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Metabotropic glutamate receptor 3 Metabotropic glutamate receptor 2 Signal Transduction MESH: Estrenes Biology MESH: Pyrrolidinones MESH: Receptors Glutamate 03 medical and health sciences Cellular and Molecular Neuroscience Organ Culture Techniques MESH: Mice Inbred C57BL Animals Humans GluD2 MESH: Receptors Metabotropic Glutamate MESH: Mice Pharmacology MESH: Humans MESH: Methoxyhydroxyphenylglycol MESH: Cerebellum MESH: Organ Culture Techniques Mice Inbred C57BL 030104 developmental biology HEK293 Cells Metabotropic glutamate receptor Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Neuropharmacology Neuropharmacology, Elsevier, 2017, 115, pp.92-99. ⟨10.1016/j.neuropharm.2016.06.001⟩ |
| ISSN: | 0028-3908 |
| DOI: | 10.1016/j.neuropharm.2016.06.001⟩ |
| Popis: | International audience; The orphan Glutamate receptor Delta2 (GluD2) intrinsic ion channel activity is indirectly triggered by glutamate through stimulation of the metabotropic glutamate receptor 1 (mGlu1), in cerebellar Purkinje cells. However, the mechanisms of GluD2 ion channel opening are entirely unknown. In this work, we investigated the signaling pathways underlying the mGlu1-induced GluD2 current, performing whole-cell voltage-clamp recordings from mGlu1 and GluD2 transfected HEK293 cells. We show that the activation of GluD2 channels via DHPG-induced mGlu1 stimulation is Gαq-dependent. Moreover, inhibition of the downstream components of the mGlu1 canonical signaling pathway PLC and PKC with U73122 and GF109203X, respectively, strongly reduced the DHPG-induced GluD2 current. These results were further confirmed on endogenous receptors at the Parallel Fiber - Purkinje Cell cerebellar synapse, indicating that the opening of the GluD2 channel by mGlu1 receptor mobilizes the canonical Gq-PLC-PKC pathway. This article is part of the Special Issue entitled 'Metabotropic Glutamate Receptors, 5 years on'. |
| Databáze: | OpenAIRE |
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