Heterologous Prime/Boost Immunization of Rhesus Monkeys by Using Diverse Poxvirus Vectors
| Autor: | Yue Sun, Valerie Philippon, Alicia Gomez-Yafal, Jenny G. Parvani, Norman L. Letvin, Phillip D. Markham, Michael S. Wyand, David C. Montefiori, Sampa Santra, Gail P. Mazzara, Dennis Panicali, Kelledy Manson |
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| Rok vydání: | 2007 |
| Předmět: |
viruses
Genetic Vectors Immunology Immunization Secondary Heterologous Vaccinia virus Antibodies Viral Virus Replication medicine.disease_cause complex mixtures Microbiology Virus chemistry.chemical_compound Virology Vaccines and Antiviral Agents medicine Animals Poxviridae Vector (molecular biology) Immunity Cellular biology Vaccination Simian immunodeficiency virus biology.organism_classification Macaca mulatta Fowlpox virus CD4 Lymphocyte Count chemistry Viral replication Insect Science RNA Viral Vaccinia |
| Zdroj: | Journal of Virology. 81:8563-8570 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.00744-07 |
| Popis: | As the diversity of potential immunogens increases within certain classes of vectors, the possibility has arisen of employing heterologous prime/boost immunizations using diverse members of the same family of vectors. The present study was initiated to explore the use of divergent pox vectors in a prime/boost regimen to elicit high-frequency cellular immune responses to human immunodeficiency virus type 1 envelope and simian immunodeficiency virus gag in rhesus monkeys. We demonstrated that monkeys vaccinated with a recombinant modified vaccinia virus Ankara (rMVA) prime/recombinant fowlpox virus (rFPV) boost regimen and monkeys vaccinated with a recombinant vaccinia virus prime/rFPV boost regimen developed comparable cellular immune responses that were greater in magnitude than those elicited by a homologous prime/boost with rMVA. Nevertheless, comparable magnitude recall cellular immune responses were observed in monkeys vaccinated with heterologous and homologous recombinant poxvirus following challenge with the CXCR4-tropic SHIV-89.6P. Consistent with this finding, comparable levels of containment of viral replication and CD4 + T-lymphocyte preservation were seen in these groups of recombinant poxvirus-vaccinated monkeys. This study supports further exploration of combining recombinant vectors of the same family in prime/boost immunization strategies to optimize vaccine-elicited cellular immune responses. |
| Databáze: | OpenAIRE |
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