GC-MS-Based Metabolomics Study of Single- and Dual-Species Biofilms of Candida albicans and Klebsiella pneumoniae
| Autor: | Marco Guida, Elisabetta de Alteriis, Angela Serena Maione, Francesco Salvatore, Anna Andolfi, Maria Michela Salvatore, Emilia Galdiero |
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| Přispěvatelé: | Galdiero, E., Salvatore, M. M., Maione, A., de Alteriis, E., Andolfi, A., Salvatore, F., Guida, M. |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Candida albican
0301 basic medicine Klebsiella pneumoniae Polymicrobial biofilm lcsh:Chemistry chemistry.chemical_compound interspecies interactions Arabitol Candida albicans lcsh:QH301-705.5 Spectroscopy Principal Component Analysis biology Chemistry Biofilm General Medicine metabolomics Corpus albicans Computer Science Applications Algorithm Leucine GC-MS polymicrobial biofilms Algorithms 030106 microbiology Metabolomic Catalysis Article Gas Chromatography-Mass Spectrometry Microbiology Inorganic Chemistry 03 medical and health sciences Metabolomics Physical and Theoretical Chemistry Least-Squares Analysis Molecular Biology Least-Squares Analysi Organic Chemistry biology.organism_classification Trehalose Culture Media 030104 developmental biology Glucose lcsh:Biology (General) lcsh:QD1-999 Biofilms Interspecies interaction |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 22, Iss 3496, p 3496 (2021) Volume 22 Issue 7 |
| ISSN: | 1422-0067 |
| Popis: | Candida albicans and Klebsiella pneumoniae frequently co-exist within the human host as a complex biofilm community. These pathogens are of interest because their association is also related to significantly increased morbidity and mortality in hospitalized patients. With the aim of highlighting metabolic shifts occurring in the dual-species biofilm, an untargeted GC-MS-based metabolomics approach was applied to single and mixed biofilms of C. albicans and K. pneumoniae. Metabolomic results showed that among the extracellular metabolites identified, approximately 40 compounds had significantly changed relative abundance, mainly involving central carbon, amino acid, vitamin, and secondary metabolisms, such as serine, leucine, arabitol, phosphate, vitamin B6, cyclo-(Phe-Pro), trehalose, and nicotinic acid. The results were related to the strict interactions between the two species and the different microbial composition in the early and mature biofilms. |
| Databáze: | OpenAIRE |
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