Changes in 45Ca and 109Cd uptake, membrane potential and cell pH in Saccharomyces cerevisiae provoked by Cd2+
| Autor: | A.P.R. Theuvenet, G. W. F. H. Borst-Pauwels, P. H. J. Peters, B.G.F. Kessels, J. Dobbelmann |
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| Rok vydání: | 1987 |
| Předmět: |
chemistry.chemical_classification
Membrane potential Cadmium Poisoning biology Saccharomyces cerevisiae Cell chemistry.chemical_element Calcium Hyperpolarization (biology) Hydrogen-Ion Concentration biology.organism_classification Microbiology Divalent Membrane Potentials Cell membrane chemistry.chemical_compound medicine.anatomical_structure chemistry Biochemistry medicine Biophysics Phosphonium Cadmium |
| Zdroj: | Journal of general microbiology. 133(4) |
| ISSN: | 0022-1287 |
| Popis: | SUMMARY: The effect of Cd2+ poisoning of Saccharomyces cerevisiae on 45Ca, 109Cd and [14C]tetraphenyl phosphonium (TPP) uptake and cell pH was examined. At Cd2+ concentrations that produced substantial K+ efflux the rates of uptake of 45Ca, 109Cd and [14C]TPP increased progressively during incubation of the cells with Cd2+, and the cell pH was lowered concomitantly. The initial rates of uptake of the divalent cations and of TPP were increased in cells pre-loaded with Cd2+, which shows that stimulation of the ion fluxes was exerted by the Cd2+ that accumulated in the cells. The distribution ratio of TPP between cells and medium, however, was decreased by Cd2+. Although hyperpolarization of the cell membrane by Cd2+ cannot be excluded, it is argued that Cd2+ primarily stimulated divalent cation uptake by increasing the cation permeability of the cell membrane allowing the cations to enter the cells more easily. |
| Databáze: | OpenAIRE |
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