E-cadherin: A determinant molecule associated with ovarian cancer progression, dissemination and aggressiveness
| Autor: | Marta Llauradó, Marina Rigau, Josep Castellví, María Florencia Abascal, Mónica H. Vazquez-Levin, Lucia Lanau, Laura Devis, José L. Sánchez, Asunción Pérez Benavente, Marina Rosso, María Laura Matos, Anna Santamaria Margalef, Jaume Reventós, Antonio Gil-Moreno, María José Besso, Lara Lapyckyj, Blanca Majem |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell Membranes Cell Culture Techniques Càncer d'ovari lcsh:Medicine Gene Expression Vimentin Biochemistry purl.org/becyt/ford/1 [https] Ovarian tumor 0302 clinical medicine Cell Movement Medicine and Health Sciences lcsh:Science Ovarian Neoplasms Multidisciplinary Tissue microarray Cell Death Messenger RNA Ascites purl.org/becyt/ford/3.1 [https] Bioquímica y Biología Molecular Cadherins Prognosis CANCER 3. Good health Nucleic acids Gene Expression Regulation Neoplastic Serous fluid Medicina Básica Cell Processes 030220 oncology & carcinogenesis Disease Progression OVARIAN NEOPLASMS purl.org/becyt/ford/3 [https] Female Anatomy Cellular Structures and Organelles CIENCIAS NATURALES Y EXACTAS Research Article CIENCIAS MÉDICAS Y DE LA SALUD Histology Cell Survival Otras Ciencias Biológicas Gastroenterology and Hepatology Biology Ciencias Biológicas 03 medical and health sciences Cytokeratin Antigens CD Ovarian cancer Cell Line Tumor medicine Genetics Biomarkers Tumor Cell Adhesion Humans Neoplasm Invasiveness RNA Messenger purl.org/becyt/ford/1.6 [https] Cadherin lcsh:R GENE EXPRESSION REGULATION Cancer Biology and Life Sciences Proteins Membrane Proteins Cell Biology medicine.disease Expressió gènica Cytoskeletal Proteins 030104 developmental biology CA-125 Antigen CADHERINS Cancer research biology.protein RNA lcsh:Q Gene expression Collagens |
| Zdroj: | PLoS ONE CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET Dipòsit Digital de la UB Universidad de Barcelona PLoS ONE, Vol 12, Iss 9, p e0184439 (2017) Recercat. Dipósit de la Recerca de Catalunya instname |
| Popis: | PLoS One. 2017 Sep 21;12(9):e0184439. doi: 10.1371/journal.pone.0184439. eCollection 2017.E-cadherin: A determinant molecule associated with ovarian cancer progression, dissemination and aggressiveness.Rosso M1, Majem B2, Devis L2, Lapyckyj L1, Besso MJ1, Llauradó M2, Abascal MF1, Matos ML1, Lanau L2, Castellví J3, Sánchez JL4, Pérez Benavente A4, Gil-Moreno A2,4, Reventós J2, Santamaria Margalef A2, Rigau M2, Vazquez-Levin MH1.Author informationAbstractOvarian cancer (OC) is the fifth cancer death cause in women worldwide. The malignant nature of this disease stems from its unique dissemination pattern. Epithelial-to-mesenchymal transition (EMT) has been reported in OC and downregulation of Epithelial cadherin (E-cadherin) is a hallmark of this process. However, findings on the relationship between E-cadherin levels and OC progression, dissemination and aggressiveness are controversial. In this study, the evaluation of E-cadherin expression in an OC tissue microarray revealed its prognostic value to discriminate between advanced- and early-stage tumors, as well as serous tumors from other histologies. Moreover, E-cadherin, Neural cadherin (N-cadherin), cytokeratins and vimentin expression was assessed in TOV-112, SKOV-3, OAW-42 and OV-90 OC cell lines grown in monolayers and under anchorage-independent conditions to mimic ovarian tumor cell dissemination, and results were associated with cell aggressiveness. According to these EMT-related markers, cell lines were classified as mesenchymal (M; TOV-112), intermediate mesenchymal (IM; SKOV-3), intermediate epithelial (IE; OAW-42) and epithelial (E; OV-90). M- and IM-cells depicted the highest migration capacity when grown in monolayers, and aggregates derived from M- and IM-cell lines showed lower cell death, higher adhesion to extracellular matrices and higher invasion capacity than E- and IE-aggregates. The analysis of E-cadherin, N-cadherin, cytokeratin 19 and vimentin mRNA levels in 20 advanced-stage high-grade serous human OC ascites showed an IM phenotype in all cases, characterized by higher proportions of N- to E-cadherin and vimentin to cytokeratin 19. In particular, higher E-cadherin mRNA levels were associated with cancer antigen 125 levels more than 500 U/mL and platinum-free intervals less than 6 months. Altogether, E-cadherin expression levels were found relevant for the assessment of OC progression and aggressiveness. Fil: Rosso, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Majem, Blanca. Universidad Autonoma de Barcelona. Hospital Vall D' Hebron. Instituto de Investigación Vall D'hebron; España Fil: Devis, Laura. Universidad Autonoma de Barcelona. Hospital Vall D' Hebron. Instituto de Investigación Vall D'hebron; España Fil: Lapyckyj, Lara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Besso, María José. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Llauradó, Marta. University of British Columbia; Canadá Fil: Abascal, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Matos, María Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Lanau, Lucia. Universidad Autonoma de Barcelona. Hospital Vall D' Hebron. Instituto de Investigación Vall D'hebron; España Fil: Castellví, Josep. Universidad Autonoma de Barcelona. Hospital Vall D' Hebron. Instituto de Investigación Vall D'hebron; España Fil: Sanchez, José Luis. Universidad Autonoma de Barcelona. Hospital Vall D' Hebron. Instituto de Investigación Vall D'hebron; España Fil: Perez Benavente, Asunción. Universidad Autonoma de Barcelona. Hospital Vall D' Hebron. Instituto de Investigación Vall D'hebron; España Fil: Gil Moreno, Antonio. Universidad Autonoma de Barcelona. Hospital Vall D' Hebron. Instituto de Investigación Vall D'hebron; España Fil: Reventós, Jaumé. Universidad Autonoma de Barcelona. Hospital Vall D' Hebron. Instituto de Investigación Vall D'hebron; España Fil: Santamaria Margalef, Anna. Universidad Autonoma de Barcelona. Hospital Vall D' Hebron. Instituto de Investigación Vall D'hebron; España Fil: Rigau, Marina. Universidad Autonoma de Barcelona. Hospital Vall D' Hebron. Instituto de Investigación Vall D'hebron; España Fil: Vazquez, Monica Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina |
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