Differential non-coding RNAs expression profiles of invasive and non-invasive pituitary adenomas
| Autor: | Chunlei Wang, Dinar Khasanov, Aamir Ahmad, Andrei Sokhatskii, Valentin Pavlov, Ilgiz Gareev, Ozal Beylerli, Elvir Valitov, Guzel Khasanova |
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| Rok vydání: | 2021 |
| Předmět: |
Invasive
QH426-470 Biochemistry Article miR-200a Pituitary adenoma microRNA Circulating Genetics Medicine In patient Molecular Biology business.industry Biochemistry (medical) Non invasive RNA lncRNA ANRIL Biomarker medicine.disease Reverse transcription polymerase chain reaction Invasive growth Cancer research Biomarker (medicine) Therapy business |
| Zdroj: | Non-coding RNA Research, Vol 6, Iss 3, Pp 115-122 (2021) Non-coding RNA Research |
| ISSN: | 2468-0540 |
| Popis: | Background Pituitary adenoma (PA) accounts for 10–15% of all intracranial neoplasms. Despite their benign nature, PA often shows invasive growth. MicroRNAs (miRNAs) and long non-coding RNAs (lncRNAs) are a class of non-coding RNAs that play important roles in PA initiation and progression. Aim The aim of this study was to find specific profiles of miR-200a and long non-coding RNA (lncRNA) antisense non-coding RNA in the INK4 locus (ANRIL) in PA based on a comparative study using Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) analyses of tumor tissue and plasma. Methods Plasma and PA tissue samples were obtained from two groups of included patients (15 invasive and 15 non-invasive PA). In addition, plasma samples from patients with invasive PA have collected pre- and post-operation. Plasma and tissue samples subjected to qRT-PCR analyses for the expression levels of miR-200a and lncRNA ANRIL. Results The expression levels of miR-200a and lncRNA ANRIL were increased in tissue samples patients with invasive PA than in the patients with non-invasive PA. In addition, the expression levels of circulating miR-200a and lncRNA ANRIL were increased in patients with invasive PA than in patients with non-invasive PA in the pre-operation period. However, the expression level of plasma circulating miR-200a and lncRNA ANRIL was decreased in patients with invasive PA in the post-operation period. Our results depicted a miR-200a and lncRNA ANRIL expression in tissue and plasma samples in the patients with invasive PA. In addition, Receiver Operating Characteristic (ROC) curve was used to evaluate the diagnostic value of these circulating miR-200a and lncRNA ANRIL. Conclusion The expression of these tumor-associated ncRNAs has been elevated in the PAs. Therefore, miR-200a and lncRNA ANRIL represents as biomarkers for diagnosis and potential targets for novel invasive PA treatment strategies. |
| Databáze: | OpenAIRE |
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