Caldicoprobacter oshimai gen. nov., sp. nov., an anaerobic, xylanolytic, extremely thermophilic bacterium isolated from sheep faeces, and proposal of Caldicoprobacteraceae fam. nov
| Autor: | Isaac D. Wagner, Juergen Wiegel, Hiroshi Yokoyama |
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| Rok vydání: | 2010 |
| Předmět: |
DNA
Bacterial Hot Temperature Molecular Sequence Data Cellobiose Biology Gram-Positive Bacteria DNA Ribosomal Microbiology Feces chemistry.chemical_compound RNA Ribosomal 16S Animals Yeast extract Anaerobiosis Raffinose Phylogeny Ecology Evolution Behavior and Systematics Base Composition Sheep Xylose Fatty Acids General Medicine 16S ribosomal RNA chemistry Tryptone Galactose Fermentation Energy source |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 60:67-71 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijs.0.011379-0 |
| Popis: | An obligately anaerobic, xylanolytic, extremely thermophilic bacterium, strain JW/HY-331T, was isolated from sheep faeces collected from a farm at the University of Georgia, USA. Cells of strain JW/HY-331T stained Gram-positive and were catalase-negative, non-motile rods. Single terminal endospores (0.4–0.6 μm in diameter) swelled the mother cell. Growth ranges were 44–77 °C (optimum 70 °C at pH70 °C 7.2) and pH70 °C 5.9–8.6 (optimum 7.2 at 70 °C). Salt tolerance was 0–2.0 % (w/v) NaCl. No growth was observed at or below 42 °C or at or above 79 °C or at pH70 °C 5.7 and below or 8.9 and above. In the presence of 0.3 % yeast extract and 0.1 % tryptone, strain JW/HY-331T utilized xylose, glucose, galactose, cellobiose, raffinose and xylan as carbon and energy sources, but not dextran, soluble potato starch, CM-cellulose, cellulose powder, casein or Casamino acids. Fermentation products from glucose were lactate, acetate, ethanol, CO2 and H2. The G+C content of the genomic DNA was 45.4 mol% (HPLC). Major cellular fatty acids were iso-C17 : 0, iso-C15 : 0 and anteiso-C17 : 0. No respiratory quinones were detected. The cell-wall structure was a single layer (Gram-type positive) of the peptidoglycan type A1γ; the cell-wall sugars were galactose and mannose. Based on 16S rRNA gene sequence analysis, ‘Catabacter hongkongensis’ HKU16 (85.4 % similarity), Caloramator fervidus ATCC 43204T (84.2 %) and Caloranaerobacter azorensis MV1087T (83.4 %) were the closest relatives, but they were only distantly related to strain JW/HY-331T. On the basis of physiological, chemotaxonomic and phylogenetic data, isolate JW/HY-331T (=DSM 21659T =ATCC BAA-1711T) is proposed as the type strain of Caldicoprobacter oshimai gen. nov., sp. nov., placed in Caldicoprobacteraceae fam. nov. within the order Clostridiales of the phylum Firmicutes. |
| Databáze: | OpenAIRE |
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