Detection of theH-RAS oncogene in human thyroid anaplastic carcinomas
| Autor: | D. Wynford-Thomas, N. Woodhouse, B. M. Stringer, J. M. Seid, P. R. Hearn, J. M. Rowson, S. Ingemansson, M. H. Parkar, M. H. Goyns |
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| Rok vydání: | 1989 |
| Předmět: |
Pathology
medicine.medical_specialty animal structures viruses Mice Nude Biology Transfection medicine.disease_cause Cell Line Mice Cellular and Molecular Neuroscience chemistry.chemical_compound medicine Animals Humans Thyroid Neoplasms Anaplastic carcinoma Molecular Biology Repetitive Sequences Nucleic Acid Southern blot Pharmacology Oncogene Carcinoma fungi Thyroid Nucleic Acid Hybridization DNA Cell Biology medicine.disease Molecular biology Cell Transformation Neoplastic Genes ras medicine.anatomical_structure chemistry Cell culture embryonic structures Molecular Medicine Carcinogenesis Neoplasm Transplantation |
| Zdroj: | Experientia. 45:372-376 |
| ISSN: | 1420-9071 0014-4754 |
| DOI: | 10.1007/bf01957483 |
| Popis: | We have transfected high-molecular-weight DNA from human thyroid carcinomas into murine 3T3 cells. As a result we identified several foci of morphologically distinct transformed cells in each of the tumour DNA transfected cultures. After a total of three rounds of transfection, the transformed cells were shown to form tumours in nude mice. Southern blot analysis of DNA prepared from third-round transfectants demonstrated the presence of human Alu repetitive sequences and, after hybridization with probes for known oncogenes, indicated the presence of the human H-RAS oncogene in 3T3 cells transfected with three out of four anaplastic carcinoma DNA samples. It appears therefore that activation of RAS genes may be an important event in the development of the anaplastic thyroid tumours. |
| Databáze: | OpenAIRE |
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