Enhanced delivery efficiency of recombinant adenovirus into tumor and mesenchymal stem cells by a novel PTD
| Autor: | Chang-Woo Lee, Jin Ht, Song My, Sung Yc, Park Sh, Lee Cg, Jin-Won Youn, Seo Sh |
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| Rok vydání: | 2008 |
| Předmět: |
Cancer Research
Genetic enhancement Biology medicine.disease_cause law.invention Adenoviridae Cell therapy Transduction (genetics) Mice law Transduction Genetic Cell Line Tumor Neoplasms medicine Animals Humans Protamines Molecular Biology Oncolytic Virotherapy Mice Inbred BALB C Mesenchymal stem cell Gene Transfer Techniques Mesenchymal Stem Cells Genetic Therapy Cytotoxicity Tests Immunologic Molecular biology Interleukin-12 Oncolytic virus Cell biology Protein Structure Tertiary Rats Recombinant DNA Molecular Medicine Ex vivo |
| Zdroj: | Cancer gene therapy. 15(11) |
| ISSN: | 1476-5500 |
| Popis: | Protein transduction domains (PTDs) are small peptides that facilitate the transduction of large molecules such as polyproteins, DNA and viruses into a eukaryotic cell. Here, we demonstrated that a novel PTD (HP4) derived from herring protamine appeared to enter C6Bu1 rat glioma cell lines more rapidly than other known PTDs such as Tat, Antp and Hph-1. Moreover, HP4 significantly enhanced in vitro transduction of recombinant adenoviruses (rAds) into various cancer cell lines, mesenchymal stem cells (MSCs) and dendritic cells, which are relatively resistant to rAd infection. Enhancement of rAd delivery into C6Bu1 and MSCs by HP4 is 20 and 7 times higher than that by Tat, respectively. The increase in the expression of rAd encoding IL-12N220L by HP4 is proportional to its antitumor effect in the ex vivo transduced mouse colon cancer model. Thus, these results suggest that HP4 could be utilized to improve the transduction efficiency of rAd, resulting in enhanced efficacy of rAd-mediated gene therapy, especially for ex vivo-transduced cell therapy. |
| Databáze: | OpenAIRE |
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