Rapid screening of N-oxides of chemical warfare agents degradation products by ESI-tandem mass spectrometry
| Autor: | Sripadi Prabhakar, L. Sridhar, Rajendiran Karthikraj, N. Prasada Raju, V. V. S. Lakshmi |
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| Rok vydání: | 2014 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Time Factors Electrospray ionization Tandem mass spectrometry Mass spectrometry Biochemistry Analytical Chemistry Isomerism Tandem Mass Spectrometry High-Throughput Screening Assays medicine Humans Sample preparation Chemical Warfare Agents Mechlorethamine Chromatography Chemistry Hydrophilic interaction chromatography Oxides Ethanolamines Triethanolamine Nitrogen Mustard Compounds Oxidation-Reduction Masking agent medicine.drug |
| Zdroj: | Analytical and Bioanalytical Chemistry. 406:5235-5241 |
| ISSN: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-014-7623-0 |
| Popis: | Rapid detection and identification of chemical warfare agents and related precursors/degradation products in various environmental matrices is of paramount importance for verification of standards set by the chemical weapons convention (CWC). Nitrogen mustards, N,N-dialkylaminoethyl-2-chlorides, N,N-dialkylaminoethanols, N-alkyldiethanolamines, and triethanolamine, which are listed CWC scheduled chemicals, are prone to undergo N-oxidation in environmental matrices or during decontamination process. Thus, screening of the oxidized products of these compounds is also an important task in the verification process because the presence of these products reveals alleged use of nitrogen mustards or precursors of VX compounds. The N-oxides of aminoethanols and aminoethylchlorides easily produce [M + H](+) ions under electrospray ionization conditions, and their collision-induced dissociation spectra include a specific neutral loss of 48 u (OH + CH2OH) and 66 u (OH + CH2Cl), respectively. Based on this specific fragmentation, a rapid screening method was developed for screening of the N-oxides by applying neutral loss scan technique. The method was validated and the applicability of the method was demonstrated by analyzing positive and negative samples. The method was useful in the detection of N-oxides of aminoethanols and aminoethylchlorides in environmental matrices at trace levels (LOD, up to 500 ppb), even in the presence of complex masking agents, without the use of time-consuming sample preparation methods and chromatographic steps. This method is advantageous for the off-site verification program and also for participation in official proficiency tests conducted by the Organization for the Prohibition of Chemical Weapons (OPCW), the Netherlands. The structure of N-oxides can be confirmed by the MS/MS experiments on the detected peaks. A liquid chromatography-mass spectrometry (LC-MS) method was developed for the separation of isomeric N-oxides of aminoethanols and aminoethylchlorides using a C18 Hilic column. Critical isomeric compounds can be confirmed by LC-MS/MS experiments, after detecting the N-oxides from the neutral loss scanning method. |
| Databáze: | OpenAIRE |
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