Detection of bovine herpesvirus 1 in cumulus-oocyte complexes of cows
| Autor: | Vanessa Lopes Dias Queiroz-Castro, José Domingos Guimarães, Abelardo Silva Júnior, Mariana Machado-Neves, Saullo Vinicius Pereira Alves, Eduardo Paulino da Costa |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Oocyte Cumulus cells Biology Immunofluorescence Immunolocalization Serology law.invention Andrology 03 medical and health sciences Confocal microscopy law medicine Confocal laser scanning microscopy Animals Herpesvirus 1 Bovine Cumulus Cells General Veterinary medicine.diagnostic_test food and beverages Infectious bovine rhinotracheitis biology.organism_classification Bovine herpesvirus 1 Reproductive failure 030104 developmental biology medicine.anatomical_structure Oocytes Cattle Female Bovine herpesvirus |
| Zdroj: | LOCUS Repositório Institucional da UFV Universidade Federal de Viçosa (UFV) instacron:UFV |
| ISSN: | 0034-5288 |
| Popis: | Bovine herpesvirus 1 (BoHV-1) is the causative agent of infectious bovine rhinotracheitis (IBR) and is also associated with reproductive failure. This study investigated the presence of BoHV-1 in cumulus-oocyte complexes (COCs) of naturally-infected cows without clinical signs of IBR. The presence of BoHV-1 in COCs was evaluated by immunofluorescence using confocal laser scanning microscopy. Blood samples and ovaries from 82 cows that had not been vaccinated against BoHV-1 were collected for serological analysis. COCs were divided into two pools: COCs derivate from seropositive cows and from seronegative cows. Then, the samples were processed for confocal microscopy analysis. The results indicated that 61% (50/82) of cows were seropositive for BoHV-1. A total of 719 COCs were obtained from the cows and processed. None of 276 COCs from the 32 seronegative cows presented BoHV-1. However, BoHV-1 was present in the cytoplasm of cumulus cells from 158 out of 443 COCs aspirated from the seropositive cows. The detection of BoHV-1 in the COCs of seropositive cows suggests that the COCs of naturally-infected, asymptomatic cows may be infected with BoHV-1. |
| Databáze: | OpenAIRE |
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