European interlaboratory comparison of breath 13CO2 analysis
| Autor: | J E Crabtree, T Shimoyama |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Quality Control
Spirillaceae Carbonates macromolecular substances Chemical Fractionation Mass Spectrometry Specimen Handling Pathogenesis Immune system Antigen Immunity Immunopathology Journal Article medicine Humans Nutritional Physiological Phenomena Carbon Isotopes biology Clinical Laboratory Techniques Gastroenterology Carbon Dioxide Helicobacter pylori bacterial infections and mycoses biology.organism_classification Europe Breath Tests Calibration Immunology Gastritis medicine.symptom Laboratories |
| Zdroj: | Gut. 43:S2-S6 |
| ISSN: | 0017-5749 |
| DOI: | 10.1136/gut.43.2008.s2 |
| Popis: | The BIOMED I programme Stable Isotopes in Gastroenterology and Nutrition (SIGN) has focused upon evaluation and standardisation of stable isotope breath tests using 13C labelled substrates. The programme dealt with comparison of 13C substrates, test meals, test conditions, analysis techniques, and calculation procedures. Analytical techniques applied for 13CO2 analysis were evaluated by taking an inventory of instrumentation, calibration protocols, and analysis procedures. Two ring tests were initiated measuring 13C abundances of carbonate materials. Evaluating the data it was found that seven different models of isotope ratio mass spectrometers (IRMS) were used by the participants applying both the dual inlet system and the continuous flow configuration. Eight different brands of certified 13C reference materials were used with a 13C abundance varying from δ13CPDB−37.2 to +2.0 ‰. CO2 was liberated from certified material by three techniques and different working standards were used varying from −47.4 to +0.4 ‰ in their δ13CPDB value. The standard deviations (SDs) found for all measurements by all participants were 0.25 ‰ and 0.50 ‰ for two carbonates used in the ring tests. The individual variation for the single participants varied from 0.02 ‰ (dual inlet system) to 0.14 ‰ (continuous flow system). The measurement of the difference between two carbonates showed a SD of 0.33 ‰ calculated for all participants. Internal precision of IRMS as indicated by the specifications of the different instrument suppliers is |
| Databáze: | OpenAIRE |
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