TIPS pentacene loaded PEO-PDLLA core-shell nanoparticles have similar cellular uptake dynamics in M1 and M2 macrophages and in corresponding in vivo microenvironments
| Autor: | Irving C. Allen, Ami Jo, Judy S. Riffle, Daniel E. Rothschild, Sheryl Coutermarsh-Ott, Michael D. Powell, Richey M. Davis, Rui Zhang, Timothy Edward Long, Dylan K. McDaniel, Veronica M. Ringel-Scaia, Kenneth J. Oestreich |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Biodistribution Materials science Polyesters Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Nanoparticle Bioengineering Inflammation Context (language use) 02 engineering and technology Article 03 medical and health sciences chemistry.chemical_compound In vivo medicine Macrophage Animals General Materials Science Organosilicon Compounds Tissue Distribution Lung Cells Cultured Drug Carriers Macrophages 021001 nanoscience & nanotechnology Asthma Mice Inbred C57BL PLGA 030104 developmental biology Biochemistry chemistry Drug delivery Biophysics Molecular Medicine Epoxy Compounds Nanoparticles medicine.symptom 0210 nano-technology |
| Zdroj: | Nanomedicine : nanotechnology, biology, and medicine. 13(3) |
| ISSN: | 1549-9642 |
| Popis: | Nanoparticle based drug delivery platforms have the potential to transform disease treatment paradigms and therapeutic strategies, especially in the context of pulmonary medicine. Once administered, nanoparticles disperse throughout the lung and many are phagocytosed by macrophages. However, there is a paucity of knowledge regarding cellular up-take dynamics of nanoparticles due largely to macrophage heterogeneity. To address this issue, we sought to better define nanoparticle up-take using polarized M1 and M2 macrophages and novel TIPS-pentacene loaded PEO-PDLLA nanoparticles. Our data reveal that primary macrophages polarized to either M1 or M2 phenotypes have similar levels of nanoparticle phagocytosis. Similarly, M1 and M2 polarized macrophages isolated from the lungs of mice following either acute (Th1) or allergic (Th2) airway inflammation also demonstrated equivalent levels of nanoparticle up-take. Together, these studies provide critical benchmark information pertaining to cellular up-take dynamics and biodistribution of nanoparticles in the context of clinically relevant inflammatory microenvironments. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect