Somatostatin 1.1 contributes to the innate exploration of zebrafish larva
| Autor: | Fanny Koëth, Claire Wyart, Maxime Kermarquer, Feng B. Quan, Lydia Djenoune, Julian Roussel, Hugo Marnas, Laura Desban, Olivier Mirat, Hervé Tostivint, François-Xavier Lejeune |
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| Přispěvatelé: | Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Physiologie moléculaire et adaptation (PhyMA), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology Mutant Video Recording lcsh:Medicine Neuropeptide Biology Article Animals Genetically Modified 03 medical and health sciences Gene Knockout Techniques 0302 clinical medicine Motor control medicine Zebrafish larvae Premovement neuronal activity Animals lcsh:Science Frameshift Mutation Zebrafish Swimming Sequence Deletion Video recording Spinal cord Multidisciplinary lcsh:R Zebrafish Proteins biology.organism_classification 3. Good health Biomechanical Phenomena 030104 developmental biology medicine.anatomical_structure Somatostatin Larva Exploratory Behavior lcsh:Q Female Neuroscience 030217 neurology & neurosurgery Locomotion |
| Zdroj: | Scientific Reports Scientific Reports, Nature Publishing Group, 2020, 10, pp.15235. ⟨10.1038/s41598-020-72039-x⟩ Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-72039-x⟩ |
| Popis: | Pharmacological experiments indicate that neuropeptides can effectively tune neuronal activity and modulate locomotor output patterns. However, their functions in shaping innate locomotion often remain elusive. For example, somatostatin has been previously shown to induce locomotion when injected in the brain ventricles but to inhibit fictive locomotion when bath-applied in the spinal cord in vitro. Here, we investigated the role of somatostatin in innate locomotion through a genetic approach by knocking out somatostatin 1.1 (sst1.1) in zebrafish. We automated and carefully analyzed the kinematics of locomotion over a hundred of thousand bouts from hundreds of mutant and control sibling larvae. We found that the deletion of sst1.1 did not impact acousto-vestibular escape responses but led to abnormal exploration. sst1.1 mutant larvae swam over larger distance, at higher speed and performed larger tail bends, indicating that Somatostatin 1.1 inhibits spontaneous locomotion. Altogether our study demonstrates that Somatostatin 1.1 innately contributes to slowing down spontaneous locomotion. |
| Databáze: | OpenAIRE |
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