Monosodium Glutamate Induces Changes in Hepatic and Renal Metabolic Profiles and Gut Microbiome of Wistar Rats
| Autor: | Amod Sharma, Jutarop Phetcharaburanin, Jarus Joonhuathon, Ubon Cha'on, Piyanard Boonnate, Carlo Selmi, Raynoo Thanan, Atit Silsirivanit, Jia V. Li, Kanokwan Nahok, Sirirat Anutrakulchai |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Monosodium glutamate microbiome Gut flora Kynurenate Kidney digestive system Article 03 medical and health sciences chemistry.chemical_compound Valine Internal medicine Sodium Glutamate medicine Animals TX341-641 Microbiome Rats Wistar 030109 nutrition & dietetics Nutrition and Dietetics biology gut microbiota Nutrition. Foods and food supply monosodium glutamate Metabolism metabolic pathway biology.organism_classification Pyridoxine metabolomics Gastrointestinal Microbiome Rats Flavoring Agents Disease Models Animal 030104 developmental biology Endocrinology medicine.anatomical_structure chemistry Liver Models Animal trimethylamine Food Science medicine.drug |
| Zdroj: | Nutrients Volume 13 Issue 6 Nutrients, Vol 13, Iss 1865, p 1865 (2021) |
| ISSN: | 2072-6643 |
| DOI: | 10.3390/nu13061865 |
| Popis: | The short- and long-term consumption of monosodium glutamate (MSG) increases urinary pH but the effects on the metabolic pathways in the liver, kidney and the gut microbiota remain unknown. To address this issue, we investigated adult male Wistar rats allocated to receive drinking water with or without 1 g% MSG for 2 weeks (n = 10, each). We performed a Nuclear Magnetic Resonance (NMR) spectroscopy-based metabolomic study of the jejunum, liver, and kidneys, while faecal samples were collected for bacterial DNA extraction to investigate the gut microbiota using 16S rRNA gene sequencing. We observed significant changes in the liver of MSG-treated rats compared to controls in the levels of glucose, pyridoxine, leucine, isoleucine, valine, alanine, kynurenate, and nicotinamide. Among kidney metabolites, the level of trimethylamine (TMA) was increased, and pyridoxine was decreased after MSG-treatment. Sequencing of the 16S rRNA gene revealed that MSG-treated rats had increased Firmicutes, the gut bacteria associated with TMA metabolism, along with decreased Bifidobacterium species. Our data support the impact of MSG consumption on liver and kidney metabolism. Based on the gut microbiome changes, we speculate that TMA and its metabolites such as trimethylamine-N-oxide (TMAO) may be mediators of the effects of MSG on the kidney health. |
| Databáze: | OpenAIRE |
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