Assessment of the Genetic Diversity of Ulex europaeus in Maui, California, Hawaii and New Zealand by a Method of Microsatellite Markers
| Autor: | Eiji Nawata, Mika Hozawa |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Genetic diversity microsatellite biology Dendrogram food and beverages Single-nucleotide polymorphism allohexaploid biology.organism_classification 01 natural sciences Ulex europaeus Analysis of molecular variance phenotypic plasticity Invasive species invasive species 03 medical and health sciences 030104 developmental biology Evolutionary biology Genotype Microsatellite genetic distances 010606 plant biology & botany |
| Zdroj: | Biology and Life Sciences Forum Volume 4 Issue 1 |
| ISSN: | 2673-9976 |
| DOI: | 10.3390/IECPS2020-08564 |
| Popis: | One of the most serious, invasive, allohexaploid plant species, Ulex europaeus, is originally from western Europe and is now spreading to the world by some unknown pathways. Plants often show phenotypic plasticity according to their environment, but elucidating the fact that the differences are derived from environmental or genetic effects is very important for further study. Thus, the aim of this study was to assess the genetic distances among Ulex europaeus from four different regions, namely Maui, California, Hawaii and New Zealand. The microsatellite method, which has been frequently used to test the genetic distances of the hexaploid plant species, was recently used for assessment because a normal single nucleotide polymorphism (SNP) often shows genotypic ambiguity on hexaploids. We tested the leaf samples of 37 mother trees from four regions (Maui: 11 California: 4 Hawaii: 7 New Zealand: 15) at five microsatellite loci. After polymerase chain reaction analyses (PCR), dinucleotide-repeat motifs (DRMs) were counted and compared to test the genetic distances of the samples. As a result, a dendrogram and analysis of molecular variance (AMOVA) showed that Ulex europaeus sampled in four different regions were genetically very close. If they show any morphological differences, they are inferred to be derived from environmental effects. |
| Databáze: | OpenAIRE |
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