Metalloproteinase-Dependent TLR2 Ectodomain Shedding is Involved in Soluble Toll-Like Receptor 2 (sTLR2) Production
| Autor: | Langjahr, P., Diaz-Jimenez, D., De La Fuente, M., Rubio, E., Golenbock, D., Bronfman, F. C., Quera, R., Lez M.-J.G., Hermoso, M.A., Benjamim C.F. |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
ADAM10
Cell Membranes lcsh:Medicine Gene Expression Ligands Biochemistry Monocytes ADAM10 Protein Animal Cells Molecular Cell Biology Medicine and Health Sciences ADAM17 Protein Receptor lcsh:Science Toll-like receptor Innate Immune System Multidisciplinary Immune System Proteins Cell biology Ectodomain Cellular Structures and Organelles Cellular Types Research Article Immune Cells Immunology Molecular Sequence Data Biology Lipopeptides Cell surface receptor Cell Line Tumor Genetics Humans Amino Acid Sequence Innate immune system lcsh:R Cell Membrane Interleukin-8 Immunity Biology and Life Sciences Proteins Membrane Proteins Cell Biology Toll-Like Receptor 2 Protein Structure Tertiary Enzyme Activation TLR2 ADAM Proteins Solubility Immune System Metalloproteases lcsh:Q Protein Translation Clinical Immunology Amyloid Precursor Protein Secretases Protein Processing Post-Translational |
| Zdroj: | PLOS ONE Artículos CONICYT CONICYT Chile instacron:CONICYT PLoS ONE PLoS ONE, Vol 9, Iss 12, p e104624 (2014) |
| Popis: | Toll-like receptor (TLR) 2, a type I membrane receptor that plays a key role in innate immunity, recognizes conserved molecules in pathogens, and triggering an inflammatory response. It has been associated with inflammatory and autoimmune diseases. Soluble TLR2 (sTLR2) variants have been identified in human body fluids, and the TLR2 ectodomain can negatively regulate TLR2 activation by behaving as a decoy receptor. sTLR2 generation does not involve alternative splicing mechanisms, indicating that this process might involve a post-translational modification of the full-length receptor; however, the specific mechanism has not been studied. Using CD14+ peripheral human monocytes and the THP-1 monocytic leukemia-derived cell line, we confirm that sTLR2 generation increases upon treatment with pro-inflammatory agents and requires a post-translational mechanism. We also find that the constitutive and ligand-induced release of sTLR2 is sensitive to pharmacological metalloproteinase activator and inhibitors leading us to conclude that metalloproteinase TLR2 shedding contributes to soluble receptor production. By expressing human TLR2 in ADAM10- or ADAM17-deficient MEF cells, we find both enzymes to be implicated in TLR2 ectodomain shedding. Moreover, using a deletion mutant of the TLR2 juxtamembrane region, we demonstrate that this domain is required for sTLR2 generation. Functional analysis suggests that sTLR2 generated by metalloproteinase activation inhibitsTLR2-induced cytokine production by this monocytic leukemia-derived cell line. The identification of the mechanisms involved in regulating the availability of soluble TLR2 ectodomain and cell surface receptors may contribute further research on TLR2-mediated processes in innate immunity and inflammatory disorders. |
| Databáze: | OpenAIRE |
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