Production and Characterization of Bacterial Cellulose with Different Nutrient Source and Surface–Volume Ratios
| Autor: | Nejla Asik, Gokhan Gunduz |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Morphology (linguistics)
Chemistry bakterijska celuloza kristaliničnost morfologija mrkva proizvodnja Forestry 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Characterization (materials science) Crystallinity chemistry.chemical_compound Nutrient Volume (thermodynamics) Chemical engineering Bacterial cellulose bacterial cellulose crystallinity morphology carrot production 0210 nano-technology |
| Zdroj: | Drvna industrija Volume 69 Issue 2 DRVNA INDUSTRIJA |
| ISSN: | 1847-1153 0012-6772 |
| Popis: | In this research, commercially available, carrot juice was explored as alternative feedstock for production of bacterial cellulose (BC) by Gluconacetobacter hansenii (ATCC® 23769™). Two types of culture media were used: Hestrin–Schramm (HS) and the carrot juice medium and these culture media were incubated statically for 10 days. The effect of different volumes of media on the microbial process and the utilization of substrates by the bacteria, were also examined. The produced BC was analyzed using X-ray diffraction (XRD), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and Fourier transform infrared spectroscopy (FT-IR). The water holding capacity (WHC) did not vary greatly with 210 mL (38.6 %), 310 mL (35.4 %), 360 mL (36.4 %) and 410 mL (37.3 %) of carrot juice media, however the WHC of 310 mL HS media (77.1 %), actually achieved a greater WHC, compared to 410 mL of HS media (55.8 %). BC produced in the carrot juice media showed higher yields than cellulose produced in HS media, with values of 1.19 g, 1.35 g, 1.33 g and 1.21 g for media with 210 mL, 310 mL, 360 mL and 410 mL, respectively. According to XRD and TGA results, there were no signifi cant differences in the crystallinity and thermal stability of cellulose produced between HS and the carrot juice medium. FT-IR of BC from HS and carrot juice medium also demonstrated a similar spectrum to alpha cellulose and microcrystalline cellulose. U radu je predstavljeno istraživanje komercijalno dostupnog soka mrkve kao alternativne sirovine za proizvodnju bakterijske celuloze (BC) uz pomoć bakterije Gluconacetobacter hansenii (ATCC 23769 ™). Primijenjene su dvije vrste medija za kulturu: Histrin-Schramm (HS) i medij od mrkvina soka te su ti mediji statički inkubirani deset dana. Istraživani su utjecaji različitih obujama medija na mikrobni proces i iskorištenje supstrata od bakterija. Dobiveni je BC analiziran s pomoću rendgenske difrakcije (XRD), skenirajuće elektronske mikroskopije (SEM), termogravimetrijske analize (TGA) i Fourierove transformirane infracrvene spektroskopije (FT-IR). Kapacitet zadržavanja vode (WHC) nije se znatnije mijenjao pri različitom obujmu medija od mrkvina soka: pri 210 mL (38,6 %), 310 mL (35,4 %), 360 mL (36,4 %) i 410 mL (37,3 %). Međutim, medij HS pri obujmu 310 mL (77,1 %) ostvario je veći WHC u usporedbi s obujmom od 410 mL (55,8 %). Proizvodnja BC-a u mediju od mrkvina soka pokazala je veće prinose od proizvodnje celuloze u mediju HS, s vrijednostima 1,19 g, 1,35 g, 1,33 g i 1,21 g za obujam medija 210 mL, 310 mL, 360 mL i 410 mL. Prema rezultatima XRD i TGA, nije bilo značajnih razlika u kristaliničnosti i toplinskoj stabilnosti proizvedene celuloze između medija HS i mrkvina soka. FT-IR analiza BC-a proizvedenoga u mediju HS i mediju od mrkvina soka također je pokazala sličan spektar alfaceluloze i mikrokristalne celuloze. |
| Databáze: | OpenAIRE |
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