Erratum to: Multiple controls affect arsenite oxidase gene expression in Herminiimonas arsenicoxydans

Autor: Odile Sismeiro, Florence Goulhen-Chollet, Philippe N. Bertin, Caroline Proux, Florence Hommais, Sandrine Koechler, Florence Arsène-Ploetze, Jean-Yves Coppé, Didier Lièvremont, Marie-Agnès Dillies, Jessica Cleiss-Arnold
Přispěvatelé: Génétique moléculaire, génomique, microbiologie (GMGM), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Microbiologie, adaptation et pathogénie (MAP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Chromatine et Régulation de la Pathogénie bactérienne (CRP), Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Dynamique, évolution et expression de génomes de microorganismes (DEEGM), Rodrigue, Agnès
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Microbiology (medical)
Transcription
Genetic
Arsenites
[SDV]Life Sciences [q-bio]
030106 microbiology
Arsenite oxidase
Molecular Sequence Data
lcsh:QR1-502
Microbiology
lcsh:Microbiology
03 medical and health sciences
Bacterial Proteins
Oxalobacteraceae
Gene expression
Operon
Amino Acid Sequence
Herminiimonas arsenicoxydans
[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology
Conserved Sequence
ComputingMilieux_MISCELLANEOUS
biology
Base Sequence
Gene Expression Profiling
Quorum Sensing
[SDV.EE.IEO] Life Sciences [q-bio]/Ecology
environment/Symbiosis
Gene Expression Regulation
Bacterial
biology.organism_classification
[SDV] Life Sciences [q-bio]
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Parasitology
Genes
Bacterial
Mutation
Erratum
Oxidoreductases
Sequence Alignment
Metabolic Networks and Pathways
[SDV.EE.IEO]Life Sciences [q-bio]/Ecology
environment/Symbiosis
Zdroj: BMC Microbiology
BMC Microbiology, BioMed Central, 2017, 17 (1), pp.74
BMC Microbiology, Vol 17, Iss 1, Pp 1-1 (2017)
ISSN: 1471-2180
Popis: Both the speciation and toxicity of arsenic are affected by bacterial transformations, i.e. oxidation, reduction or methylation. These transformations have a major impact on environmental contamination and more particularly on arsenic contamination of drinking water. Herminiimonas arsenicoxydans has been isolated from an arsenic- contaminated environment and has developed various mechanisms for coping with arsenic, including the oxidation of As(III) to As(V) as a detoxification mechanism.In the present study, a differential transcriptome analysis was used to identify genes, including arsenite oxidase encoding genes, involved in the response of H. arsenicoxydans to As(III). To get insight into the molecular mechanisms of this enzyme activity, a Tn5 transposon mutagenesis was performed. Transposon insertions resulting in a lack of arsenite oxidase activity disrupted aoxR and aoxS genes, showing that the aox operon transcription is regulated by the AoxRS two-component system. Remarkably, transposon insertions were also identified in rpoN coding for the alternative N sigma factor (sigma54) of RNA polymerase and in dnaJ coding for the Hsp70 co-chaperone. Western blotting with anti-AoxB antibodies and quantitative RT-PCR experiments allowed us to demonstrate that the rpoN and dnaJ gene products are involved in the control of arsenite oxidase gene expression. Finally, the transcriptional start site of the aoxAB operon was determined using rapid amplification of cDNA ends (RACE) and a putative -12/-24 sigma54-dependent promoter motif was identified upstream of aoxAB coding sequences.These results reveal the existence of novel molecular regulatory processes governing arsenite oxidase expression in H. arsenicoxydans. These data are summarized in a model that functionally integrates arsenite oxidation in the adaptive response to As(III) in this microorganism.
Databáze: OpenAIRE
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