Distinctive protein expression patterns of the strain Brevundimonas sp. ZF12 isolated from the aqueous zone containing high levels of radiation to cadmium-induced stress
| Autor: | Hossein Shahbani Zahiri, Hamidreza Hajfarajollah, Leila Alidoust, Nasrin Masoudzadeh, Nima Samie, Kambiz Akbari Noghabi, Sima Modiri, Hakimeh Sharafi, Hojatollah Vali |
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| Rok vydání: | 2014 |
| Předmět: |
Proteome
DNA repair chemistry.chemical_element Bioengineering Biology Proteomics medicine.disease_cause Applied Microbiology and Biotechnology Hot Springs Mass Spectrometry Bacterial Proteins medicine Electrophoresis Gel Two-Dimensional Polyacrylamide gel electrophoresis Cadmium Strain (chemistry) Brevundimonas Caulobacteraceae General Medicine Metabolism biology.organism_classification Oxidative Stress Biochemistry chemistry Oxidative stress Biotechnology |
| Zdroj: | Journal of Biotechnology. 186:49-57 |
| ISSN: | 0168-1656 |
| Popis: | In the current study, different protein expression profiles in a strain Brevundimonas sp. ZF12, isolated from the aqueous zone containing high levels of radiation, were characterized following exposure to cadmium (II) using a proteomic strategy. In order to gain a deeper understanding of the cellular events that allow this strain to survive and undergo cadmium adaptation and sorption, the strain was tested under three experimental conditions of 5, 10 and 30 ppm cadmium (II) ions stress. Two-dimensional polyacrylamide gel electrophoresis and mass spectrometry were used to identify the differentially expressed proteins under cadmium (II) stress. 20 differentially expressed spots were successfully identified by MS/MS analysis. These proteins are involved in DNA repair and protection, amino acid metabolism, nucleotide metabolism, energy homeostasis, oxidative stress response, redox homeostasis, protein folding and heat-shock response. The results obviously indicate that the ZF12 strain tends to endure the cadmium (II) stress conditions by modification in many aspects of its cellular physiology and metabolism. |
| Databáze: | OpenAIRE |
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